Purification and characterization of ferritin from alfalfa seeds

被引：19

作者：

Barcelo, F

Miralles, F

Arean, CO

机构：

[1] UNIV ISLAS BALEARES,DEPT QUIM,PALMA DE MALLORCA 07071,SPAIN

[2] UNIV ISLAS BALEARES,DEPT BIOL FUNDAMENTAL & CIENCIAS SALUD,PALMA DE MALLORCA 07071,SPAIN

来源：

JOURNAL OF INORGANIC BIOCHEMISTRY | 1997年 / 66卷 / 01期

关键词：

D O I：

10.1016/S0162-0134(96)00155-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ferritin from alfalfa (Medicago sativa) seeds was isolated, purified, and characterized The apparent molecular mass of the native protein was found to be 560 kDa. Electrophoresis in denaturing gradient polyacrylamide-SDS gels revealed subunits of 28-26.5 kDa. The average iron cores were 4 nm in diameter and contained about 1400 iron atoms, with an iron-to-phosphorus ratio of 4:1. N-terminal amino acid sequencing of the 28 kDa subunit revealed close homology with other plant proteins. Immunochemical analysis using polyclonal antibodies raised against pea-seed ferritin has confirmed, in agreement with previous reports, that plant proteins share common epitopes. (C) 1997 Elsevier Science Inc.

引用

页码：23 / 27

页数：5

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