Spatial and temporal variations in chitinolytic gene expression and bacterial biomass production during chitin degradation

被引:55
作者
Baty, AM
Eastburn, CC
Techkarnjanaruk, S
Goodman, AE
Geesey, GG
机构
[1] Montana State Univ, Ctr Biofilm Engn, Bozeman, MT 59717 USA
[2] Montana State Univ, Dept Microbiol, Bozeman, MT 59717 USA
[3] Flinders Univ S Australia, Sch Biol Sci, Adelaide, SA 5001, Australia
关键词
D O I
10.1128/AEM.66.8.3574-3585.2000
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Growth of the chitin-degrading marine bacterium S91 on solid surfaces under oligotrophic conditions was accompanied by the displacement of a large fraction of the surface-derived bacterial production into the flowing bulk aqueous phase, irrespective of the value of the surface as a nutrient source, Over a 200-h period of surface colonization, 97 and 75% of the bacterial biomass generated on biodegradable chitin and a nonnutritional silicon surface, respectively, detached to become part of the free-living population in the bulk aqueous phase. Specific surface-associated growth rates that included the cells that subsequently detached from the substrata varied depending on the nutritional value of the substratum and during the period of surface colonization. Specific grow-th fates of 3.79 and 2.83 day(-1) were obtained when cells first began to proliferate on a pure chitin film and a silicon surface, respectively. Later, when cell densities on the surface and detached cells as CFU in the bulk aqueous phase achieved a quasi-steady state, specific growth rates decreased to 1.08 and 0.79 day(-1) on the chitin and silicon surfaces, respectively, Virtually all of the cells that detached from either the chitin or the silicon surfaces and the majority of tells associated with the chitin surface over the 200-h period of surface colonization displayed no detectable expression of the chitin-degrading genes chiA and chiB. Cells displaying high levels of chiA-chiB expression were detected only on the chitin surface and then only clustered in discrete areas of the surface. Surface-associated, differential gene expression and displacement of bacterial production from surfaces represent adaptations at the population level that promote efficient utilization of limited resources and dispersal of progeny to maximize access to new sources of energy and maintenance of the population.
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收藏
页码:3574 / 3585
页数:12
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