Relationship between CD38 expression on peripheral blood T-cells and monocytes, and response to antiretroviral therapy: A one-year longitudinal study of a cohort of chronically infected ART-naive HIV-1+patients

被引:24
作者
Almeida, Maria
Cordero, Miguel
Almeida, Julia
Orfao, Alberto
机构
[1] Univ Salamanca, Ctr Canc Res, Serv Gen Citometria, Serv Flow Cytometry, Salamanca 37007, Spain
[2] Univ Salamanca, Dept Med, E-37008 Salamanca, Spain
[3] Univ Hosp Salamanca, Serv Internal Med Unity Infect Dis, Salamanca, Spain
关键词
CD38; expression; HIV-1; antiretroviral therapy; monocytes; T-lymphocytes; disease progression; immune activation; hierarchical clustering analysis;
D O I
10.1002/cyto.b.20144
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Background: HIV-1 infection has been associated with high expression of CD38 on peripheral blood (PB) CDB+ and CD4+ T-cells, which has been related with poor prognosis in untreated HIV-1+ patients. In turn, CD38 expression on PB monocytes from HIV-1+ individuals and its behavior after starting antiretroviral therapy (ART) have been poorly studied. Methods: CD38 expression on PB CD8+ and CD4+ T-lymphocytes and monocytes was prospectively analyzed in 30 ART-naive HIV-1+ patients, using a quantitative multiparameter flow cytometry approach. Patients were tested prior to therapy, and at weeks +2, +4, +8, +12, and +52 after ART. Results: Prior to ART, CD38 expression was significantly increased on PB CDB+ and CD4+ T-cells and monocytes; despite a significant decrease after ART, CD38 expression remained abnormally high on PB CDB+ T-cells and monocytes, even after one year of therapy, in the absence of detectable plasma viral load. The ART-induced early changes on CD38 expression by PB T-cells and monocytes differed among the cell subsets analyzed and patient groups, probably reflecting an interaction between the direct effects of therapy and a redistribution of the PB compartments of T-cells and monocytes. Hierarchical clustering analysis showed that the overall pattern of changes in CD38 expression observed early after starting ART was predictive of a better response to therapy, not only for PB CDB+ T-cells, but also for CD4+ T-cells and monocytes. Accordingly, those HIV-1+ patients, who experienced a more pronounced increase in CD38 expression on both PB CD4+ T-cells and monocytes after 2 weeks of ART, showed a more rapid viral clearance, which might reflect decreased HIV-1 replication in lymph nodes and other tissues, and a partial restoration of hematopoiesis. Conclusions: Combined quantitative measurement of CD38 expression on PB monocytes, and CDB+ and CD4+ T-cells is a more useful tool for monitoring HIV-1+ patients under ART, rather than quantitation of CD38 expression on PB CD8+ T-lymphocytes alone. (c) 2006 Clinical Cytometry Society.
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页码:22 / 33
页数:12
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