Proliferative activity and expression of cyclin-dependent kinase inhibitor p21WAF1 and p53 protein in endothelial cells of human aorta during replicative aging in vitro

被引：6

作者：

Antropova, YG ^{[1
]}

Bryantsev, AL ^{[1
]}

Kalinina, NI ^{[1
]}

Il'inskaya, OP ^{[1
]}

Tararak, EM ^{[1
]}

机构：

[1] Russian Minist Hlth, Russian Res & Prod Ctr Cardiol, Inst Expt Cardiol, Lab Mol & Cellular Cardiol, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2002年 / 134卷 / 01期

关键词：

human aortic endothelial cells; proliferation aging; atherogenesis; p53; p21(WAF1);

D O I：

10.1023/A:1020677209755

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 [基础医学];

摘要：

Experiments with bromodeoxyuridine showed that the count of nonproliferating cells in a monolayer culture of aortic endothelial cell, tom adult humans rapidly increased during longterm subculturing. Cytochemical assay showed that these cells contain neutral beta-galactosidase, the marker of aging cells. Immunocytochemical assay demonstrated that most cells express p53. protein and inhibitor of the cell cycle p21(WAFI).

引用

页码：81 / 83

页数：3

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