Calcium phosphate coating containing silver shows high antibacterial activity and low cytotoxicity and inhibits bacterial adhesion

被引：76

作者：

Ando, Yoshiki ^{[1
,2
]}

Miyamoto, Hiroshi ^{[1
]}

Noda, Iwao ^{[2
]}

Sakurai, Nobuko ^{[2
]}

Akiyama, Tomonori ^{[1
]}

Yonekura, Yutaka ^{[3
]}

Shimazaki, Takafumi ^{[3
]}

Miyazaki, Masaki ^{[3
]}

Mawatari, Masaaki ^{[3
]}

Hotokebuchi, Takao ^{[3
]}

机构：

[1] Saga Univ, Fac Med, Dept Pathol & Microbiol, Div Microbiol, Saga 8498501, Japan

[2] Japan Med Mat Corp, Res Dept, Yodogawa Ku, Osaka 5320003, Japan

[3] Saga Univ, Fac Med, Dept Orthopaed Surg, Saga 8498501, Japan

来源：

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2010年 / 30卷 / 01期

关键词：

Infection; Antibacterial; Bacterial adherence; Cytotoxicity; Orthopaedic implant; STAPHYLOCOCCUS-EPIDERMIDIS; WOUND-INFECTION; HYDROXYAPATITE; TITANIUM; MODEL; BIOMATERIALS; IMPLANTS; EFFICACY; OUTCOMES; SYSTEM;

D O I：

10.1016/j.msec.2009.09.015

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

082905 [生物质能源与材料]; 100103 [病原生物学];

摘要：

Surgical site infection is one of the serious complications of orthopedic implants. In order to reduce the incidence of implant-associated infections, we developed a novel coating technology of calcium phosphate (CP) containing silver (Ag). designated Ag-CP coating, using a thermal spraying technique. In this study, we evaluated the antibacterial efficacy and biological safety of this coating. In vitro antibacterial activity tests showed that the growths of Escherichia coli, Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) are completely suppressed on Ag-CP coating. In vitro bacterial adherence tests revealed that the number of adherent bacteria on the surface of this coating is significantly less (p<0.02) than that on the surface of the CP coating. Moreover, the Ag-CP coating completely inhibits MRSA adhesion [<10 colony-forming units (CFU)] when 10(2) CFU MRSA is inoculated. On the other hand, V79 Chinese hamster lung cells were found to grow on the Ag-CP coating as well as on the CP coating in a cytotoxicity test. These results indicate that the Ag-CP coating on the surface of orthopedic implants exhibits antibacterial activity and inhibits bacterial adhesion without cytotoxicity. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：175 / 180

页数：6

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