Expression of a fungal cellobiohydrolase in insect cells

被引：11

作者：

vonOssowski, I

Teeri, T

Kalkkinen, N

OkerBlom, C

机构：

[1] VTT BIOTECHNOL & FOOD RES, FIN-02044 ESPOO, FINLAND

[2] UNIV HELSINKI, INST BIOTECHNOL, FIN-00014 HELSINKI, FINLAND

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 233卷 / 01期

基金：

芬兰科学院;

关键词：

D O I：

10.1006/bbrc.1997.6391

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene for Trichoderma reesei cellobiohydrolase I (CBHI) was expressed with a recombinant baculovirus and high levels of secreted protein were produced in Spodoptera frugiperda and Trichoplusia ni insect cells. Electrophoretic analysis indicated that the recombinant CBHI (rCBHI) was similar in apparent molecular weight to the native form and immunoblotting with anti-CBHI monoclonal antibodies confirmed its identity. The rCBHI was easily purified by affinity and hydrophobic interaction chromatography and demonstrated enzymatic activity on soluble substrate. (C) 1997 Academic Press.

引用

收藏

页码：25 / 29

页数：5

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