DNA-ligand interaction detected by proton selective and non-selective spin-lattice relaxation rate analysis

被引：17

作者：

Bonechi, C ^{[1
]}

Donati, A ^{[1
]}

Picchi, MP ^{[1
]}

Rossi, C ^{[1
]}

Tiezzi, E ^{[1
]}

机构：

[1] UNIV SIENA,DEPT CHEM,I-53100 SIENA,ITALY

来源：

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS | 1996年 / 115卷

关键词：

DNA-ligand interaction; proton selective and non-selective spin-lattice relaxation rate analysis;

D O I：

10.1016/0927-7757(96)03659-X

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

In this paper, a method based on NMR measurements for studying the ability of ligands to interact with macromolecule surfaces is proposed. It is based on temperature-dependent NMR proton selective and non-selective spin-lattice relaxation rate experiments and assumes fast chemical exchange conditions of the ligand between free and bound states. The results obtained appear able to identify DNA-psoralen complex formation. The use of this methodology for monitoring non-covalent interaction of ligands and bipolymers is suggested.

引用

页码：89 / 95

页数：7

共 14 条

[1] INFLUENCE OF CROSS-RELAXATION ON NMR SPIN-LATTICE RELAXATION-TIMES [J].