Characterization of an Arabidopsis thaliana cDNA encoding an S-adenosylmethionine-sensitive threonine synthase - Threonine synthase from higher plants

被引：46

作者：

Curien, G ^{[1
]}

Dumas, R ^{[1
]}

Ravanel, S ^{[1
]}

Douce, R ^{[1
]}

机构：

[1] RHONE POULENC AGROCHIM,CNRS UMR 41,LAB MIXTE,F-69263 LYON,FRANCE

来源：

FEBS LETTERS | 1996年 / 390卷 / 01期

关键词：

threonine synthase; o-phospho-L-homoserine; S-adenosylmethionine; pyridoxal 5'-phosphate; chloroplast; Arabidopsis thaliana;

D O I：

10.1016/0014-5793(96)00633-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An Arabidopsis thaliana cDNA encoding an S-adenosylmethionine-sensitive threonine synthase (EC 4.2.99.2) has been isolated by functional complementation of an Escherichia coli mutant devoid of threonine synthase activity. Threonine synthase from A. thaliana was shown to be synthesized with a transit peptide. The recombinant protein is activated by S-adenosylmethionine in the same range as the plant threonine synthase and evidence is presented for an involvement of the N-terminal part of the mature enzyme in the sensitivity to S-adenosylmethionine.

引用

页码：85 / 90

页数：6

共 31 条

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