NK Cells Recognize and Kill Human Glioblastoma Cells with Stem Cell-Like Properties

被引：273

作者：

Castriconi, Roberta ^{[2
]}

Daga, Antonio ^{[3
]}

Dondero, Alessandra ^{[2
]}

Zona, Gianluigi ^{[4
]}

Poliani, Pietro Luigi ^{[5
]}

Melotti, Alice ^{[3
,6
]}

Griffero, Fabrizio ^{[3
,6
]}

Marubbi, Daniela ^{[3
,6
]}

Spaziante, Renato ^{[4
]}

Bellora, Francesca ^{[2
]}

Moretta, Lorenzo ^{[1
,2
,7
]}

Moretta, Alessandro ^{[2
,7
]}

Corte, Giorgio ^{[3
,6
]}

Bottino, Cristina ^{[1
,2
]}

机构：

[1] Ist Giannina Gaslini, Clin & Expt Immunol Lab, I-16148 Genoa, Italy

[2] Univ Genoa, Dipartimento Med Sperimentale, Genoa, Italy

[3] Ist Nazl Ric Canc, I-16132 Genoa, Italy

[4] Univ Genoa, Dipartimento Neurosci Oftalmol & Genet, Genoa, Italy

[5] Univ Brescia, Serv Anat Patol 1, Brescia, Italy

[6] Univ Brescia, Dipartimento Oncol Biol & Genet, Brescia, Italy

[7] Univ Brescia, Ctr Eccellenza Ric Biomed, Brescia, Italy

来源：

JOURNAL OF IMMUNOLOGY | 2009年 / 182卷 / 06期

关键词：

HUMAN NATURAL-KILLER; CLASS-I MOLECULES; IMMUNE-RESPONSE; GLIOMA-CELLS; POLIOVIRUS RECEPTOR; NERVOUS-SYSTEM; EXPRESSION; LIGANDS; GROWTH; IMMUNOTHERAPY;

D O I：

10.4049/jimmunol.0802845

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In this study, cancer cells were isolated from tumor specimens of nine glioblastoma patients. Glioblastoma cells, cultured under suitable culture conditions, displayed markers typical of neural stem cells, were capable of partial multilineage differentiation in vitro, and gave origin to infiltrating tumors when orthotopically injected in NOD/SCID mice. These cells, although resistant to freshly isolated NK cells, were highly susceptible to lysis mediated by both allogeneic and autologous IL-2 (or IL-15)-activated NK cells. Indeed, all stem cell-cultured glioblastoma cells analyzed did not express protective amounts of HILA class I molecules, while expressing various ligands of activating NK receptors that triggered optimal NK cell cytotoxicity. Importantly, glioblastoma stem cells expressed high levels of PVR and Nectin-2, the ligands of DNAM-1-activating NK receptor. The Journal of Immunology, 2009,182: 3530-3539.

引用

页码：3530 / 3539

页数：10

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