Rapid uncoupling of oxidative phosphorylation accompanies glutamate toxicity in rat cerebellar granule cells

被引：47

作者：

Atlante, A

Gagliardi, S

Minervini, GM

Marra, E

Passarella, S

Calissano, P

机构：

[1] CNR, IST NEUROBIOL, I-00137 ROME, ITALY

[2] CNR, CTR STUDIO MITOCONDRI & METAB ENERGET, I-70126 BARI, ITALY

[3] UNIV MOLISE, DIPARTIMENTO SAVA, CAMPOBASSO, ITALY

来源：

NEUROREPORT | 1996年 / 7卷 / 15-17期

关键词：

neurotoxicity; glutamate; cerebellar granule cells; mitochondria; bioenergetics;

D O I：

10.1097/00001756-199611040-00023

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A 100 mu M glutamate pulse administered to rat cerebellar granule cells causes a very rapid and progressive decrease in both cell and mitochondrial oxygen consumption caused by glucose and succinate addition, respectively. The respiratory control ratio, which reflects the ability of mitochondria to produce ATP, is reduced by 50% within the first 30 min after glutamate addition. Subsequent to glutamate exposure, a progressive decrease of respiratory control ratio to almost 1 was found within the following 3-5 h. The addition of extra calcium had no effect per se on oxygen consumption by cell homogenate.

引用

页码：2519 / 2523

页数：5

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