193 nm photodissociation of larger multiply-charged biomolecules

被引：97

作者：

Guan, ZQ ^{[1
]}

Kelleher, NL ^{[1
]}

OConnor, PB ^{[1
]}

Aaserud, DJ ^{[1
]}

Little, DP ^{[1
]}

McLafferty, FW ^{[1
]}

机构：

[1] CORNELL UNIV, BAKER LAB CHEM, ITHACA, NY 14853 USA

来源：

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY AND ION PROCESSES | 1996年 / 157卷

关键词：

Fourier transform ion cyclotron resonance; electrospray; photodissociation; 193; nm;

D O I：

10.1016/S0168-1176(96)04399-6

中图分类号：

O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];

学科分类号：

070203 ; 070304 ; 081704 ; 1406 ;

摘要：

In contrast to most ion dissociation methods, 193 nm ultraviolet photodissociation of electrosprayed melittin (2.8 kDa) and ubiquitin (8.6 kDa) molecular ions yields new c and z ions (backbone amine bond dissociation) that provide additional sequence information. Dissociation by collisions or infrared photons produce b and y ions; for cleavages between the same amino acids the c ion represents the addition of NH2 to the b ion, and z the loss of NH2 from the y ion, so that these ions can be differentiated by this +/- 16.02 Da difference. However, 193 nm photodissociation of 12-29 kDa ions as yet does not give collectable product ions, and that of the very stable y(18)(2+) ion from ubiquitin only effects a side chain loss. 193 nm irradiation of negative ions of all-T 30-mer DNA appears to eject electrons; apparently this is the first observation of electron photodetachment from multiply-charged negative ions.

引用

页码：357 / 364

页数：8

共 38 条

[1] DISTINGUISHING N-TERMINUS AND C-TERMINUS IONS FOR MASS-SPECTROMETRY SEQUENCING OF PROTEINS WITHOUT PRIOR DEGRADATION [J].