Effect of growth factors on the expression of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase in rat-1 fibroblasts

被引:14
作者
Joaquin, M
Salvado, C
Bellosillo, B
Lange, AJ
Gil, J
Tauler, A
机构
[1] UNIV BARCELONA,UNIT BIOQUIM,DEPT CIENCIES FISIOL HUMANES & NUTR,E-08028 BARCELONA,SPAIN
[2] UNIV MINNESOTA,DEPT BIOCHEM,MINNEAPOLIS,MN 55455
关键词
D O I
10.1074/jbc.272.5.2846
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The activation of glycolytic flux is a biochemical characteristic of growing cells. Several reports have demonstrated the role of fructose 2,6-bisphosphate in this process. In this paper we show that the levels of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (6PF2K/Fru-2,6-P(2)ase) mRNA are modulated in response to serum and growth factors and this effect is due to regulation of its transcription rate. The modulation of the expression of this enzyme by growth factors differs according their mitogenic effect; both lysophosphatidic acid and epidermal growth factor, when added alone, increased the mRNA levels, but endothelin had no effect. Furthermore, cAMP, which acts as an antimitogenic signal in Rat-1 fibroblasts, produced a decrease in 6PF2K/ Fru-2,6-P(2)ase mRNA and inhibited the effects of lysophosphatidic acid and epidermal growth factor on 6PF2K/Fru-2,6-P(2)ase expression. PD 098059, a specific inhibitor of the activation of the mitogen-activated protein kinase, was able to prevent the effect of EGF on 6PF2K/Fru-2,6-P(2)ase gene expression. These results imply that activation of mitogen-activated protein kinase is required for the stimulation of the transcription of 6PF2K/Fru-2,6-P(2)ase by EGF.
引用
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页码:2846 / 2851
页数:6
相关论文
共 58 条
[1]   TRANSFORMING P21(RAS) MUTANTS AND C-ETS-2 ACTIVATE THE CYCLIN D1 PROMOTER THROUGH DISTINGUISHABLE REGIONS [J].
ALBANESE, C ;
JOHNSON, J ;
WATANABE, G ;
EKLUND, N ;
VU, D ;
ARNOLD, A ;
PESTELL, RG .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (40) :23589-23597
[2]  
AUFFRAY C, 1980, EUR J BIOCHEM, V107, P303
[3]  
AUSEBEL FM, 1994, CURRENT PROTOCOLS MO
[4]   EFFECTS OF EPIDERMAL GROWTH-FACTOR ON GLYCOLYSIS IN A431 CELLS [J].
BAULIDA, J ;
ONETTI, R ;
BASSOLS, A .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 183 (03) :1216-1223
[5]  
BERGERS G, 1995, MOL CELL BIOL, V15, P3748
[6]   PHORBOL 12-MYRISTATE 13-ACETATE AND INSULIN INCREASE THE CONCENTRATION OF FRUCTOSE 2,6-BISPHOSPHATE AND STIMULATE GLYCOLYSIS IN CHICKEN-EMBRYO FIBROBLASTS [J].
BOSCA, L ;
ROUSSEAU, GG ;
HUE, L .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (19) :6440-6444
[7]  
BOSCA L, 1986, BIOCHEM J, V236, P596
[8]  
Bradford MM, 1976, ANAL BIOCHEM, V112, P195
[9]   ADENYLATE-CYCLASE STIMULATING AGENTS AND MITOGENS RAISE FRUCTOSE 2,6-BISPHOSPHATE LEVELS IN HUMAN-FIBROBLASTS - EVIDENCE FOR A DUAL CONTROL OF THE METABOLITE [J].
BRUNI, P ;
VASTA, V ;
FARNARARO, M .
FEBS LETTERS, 1987, 222 (01) :27-31
[10]   REGULATION OF FRUCTOSE 2,6-BISPHOSPHATE METABOLISM IN HUMAN-FIBROBLASTS [J].
BRUNI, P ;
VASTA, V ;
FARNARARO, M .
BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 887 (01) :23-28