High-resolution mosaic imaging with multifocal, multiphoton photon-counting microscopy

被引：16

作者：

Chandler, Eric ^{[1
]}

Hoover, Erich ^{[1
]}

Field, Jeff ^{[1
]}

Sheetz, Kraig ^{[1
]}

Amir, Wafa ^{[1
]}

Carriles, Ramon ^{[1
]}

Ding, Shi-you ^{[2
]}

Squier, Jeff ^{[1
]}

机构：

[1] Colorado Sch Mines, Ctr Microintegrated Opt Adv Biol Control, Dept Phys, Golden, CO 80401 USA

[2] Natl Renewable Energy Lab, Golden, CO 80401 USA

来源：

APPLIED OPTICS | 2009年 / 48卷 / 11期

关键词：

REAL-TIME; 2-PHOTON; OSCILLATOR; EXCITATION; SINGLE; POWER;

D O I：

10.1364/AO.48.002067

中图分类号：

O43 [光学];

学科分类号：

070207 [光学];

摘要：

High-resolution mosaic imaging is performed for the first time to our knowledge with a multifocal, multi-photon, photon-counting imaging system. We present a novel design consisting of a home-built femtosecond Yb-doped KGdWO4 laser with an optical multiplexer, which is coupled with a commercial Olympus IX-71 microscope frame. Photon counting is performed using single-element detectors and an inexpensive electronic demultiplexer and counters. (C) 2009 Optical Society of America

引用

页码：2067 / 2077

页数：11

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