Severe acute respiratory syndrome coronavirus accessory protein 9b is a virion-associated protein

被引：45

作者：

Xu, Ke ^{[1
,2
]}

Zheng, Bo-Jian ^{[3
]}

Zeng, Rong ^{[4
]}

Lu, Wei ^{[1
,2
]}

Lin, Yong-Ping ^{[3
]}

Xue, Liang ^{[1
]}

Li, Li ^{[2
]}

Yang, Lei-Lei ^{[2
]}

Xu, Chen ^{[2
]}

Dai, Jie ^{[4
]}

Wang, Fei ^{[2
]}

Li, Qing ^{[2
]}

Dong, Qing-Xi ^{[1
]}

Yang, Rui-Fu ^{[5
]}

Wu, Jia-Rui ^{[1
]}

Sun, Bing ^{[1
,2
,6
]}

机构：

[1] Chinese Acad Sci, Mol Cell Biol Lab, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China

[2] Chinese Acad Sci, Mol Virol Lab, Inst Pasteur Shanghai, Shanghai Inst Biol Sci, Shanghai 200025, Peoples R China

[3] Univ Hong Kong, Queen Mary Hosp, Dept Microbiol, Hong Kong, Hong Kong, Peoples R China

[4] Chinese Acad Sci, Res Ctr Proteome Anal Key Lab Prote, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China

[5] Acad Mil Med Sci, Inst Microbiol & Epidemiol, Beijing 100071, Peoples R China

[6] Shanghai Univ E Inst, Div Immunol, Shanghai, Peoples R China

来源：

VIROLOGY | 2009年 / 388卷 / 02期

基金：

中国国家自然科学基金;

关键词：

SARS-CoV; ORF9b; Virion-associated protein; SARS-ASSOCIATED CORONAVIRUS; VIRAL STRUCTURAL PROTEIN; VIRUS-INFECTED CELLS; AUG INITIATOR CODON; OPEN READING FRAME; 3A PROTEIN; EXPRESSING CELLS; 7A PROTEIN; TRANSLATION; PARTICLES;

D O I：

10.1016/j.virol.2009.03.032

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Eight accessory proteins have been identified in severe acute respiratory syndrome-associated coronavirus (SARS-CoV). They are believed to play roles in the viral life cycle and may contribute to the pathogenesis and virulence. ORF9b as one of these accessory proteins is located in subgenomic mRNA9 and encodes a 98 amino acid protein. However, whether 9b protein is a Structural component of SARS-CoV particles remains unknown. In this study, we demonstrate that 9b protein is translated from bicistronic mRNA9 via leaky ribosome scanning and it is incorporated into both virus-like particles (VLPs) and purified SARS-CoV virions. Further analysis shows that sufficient incorporation of 9b protein into VLPs is dependent upon the co-expression of E and M proteins, but not upon the presence of either S or N protein. Our data indicate that 9b protein of SARS-CoV is another virion-associated accessory protein. This finding will lead to a better understanding of the properties of the SARS-CoV 9b protein. (c) 2009 Elsevier Inc. All rights reserved.

引用

页码：279 / 285

页数：7

共 43 条

[1] Coronaviral hypothetical and structural proteins were found in the intestinal surface enterocytes and pneumocytes of severe acute respiratory syndrome (SARS) [J].