Survival and growth of Burkholderia cepacia within the free-living amoeba Acanthamoeba polyphaga

被引：49

作者：

Landers, P

Kerr, KG ^{[1
]}

Rowbotham, TJ

Tipper, JL

Keig, PM

Ingham, E

Denton, M

机构：

[1] Univ Leeds, Dept Microbiol, Leeds LS2 9JT, W Yorkshire, England

[2] Publ Hlth Lab, Leeds LS15 7TR, W Yorkshire, England

[3] Gen Infirm, Dept Microbiol, Leeds LS1 3EX, W Yorkshire, England

来源：

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 2000年 / 19卷 / 02期

关键词：

D O I：

10.1007/s100960050442

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The aim of this study was to investigate whether Burkholderia cepacia is capable of survival and growth within the free-living amoeba Acanthamoeba polyphaga using a differential immunofluorescence assay of bacterial-amoebal cocultures and viable counts of bacteria determined after amoebal lysis. The numbers of intra-amoebal bacteria and the numbers of infected amoebae increased over time; although, when heat-killed bacteria were used, no intracellular bacteria were observed, These findings should be taken into account in future studies of environmental reservoirs of Burkholderia cepacia.

引用

页码：121 / 123

页数：3

共 15 条

[1] Invasion of respiratory epithelial cells by Burkholderia (Pseudomonas) cepacia [J].