ICAM-2 has been implicated in leukocyte transmigration in vitro, but there is little in vivo evidence to support this. To address this, neutrophil migration was investigated in ICAM-2-deficient mice (KO) and in wild-type (WT) mice treated with an anti-ICAM-2 blocking monoclonal antibody (mAb) (3C4). In a peritonitis model, IL-10-induced accumulation of neutrophils was significantly reduced in mice treated with 3C4 (51% inhibition) and in KO mice (41% inhibition). In contrast, TNF-alpha- or thioglycolate-induced responses were not suppressed in KO mice. Analysis of IL-1 beta-induced leukocyte responses in cremasteric venules of KO animals by intravital microscopy indicated a defect in transmigration (44% inhibition) but not rolling oradhesion. Wound before, TNF-alpha-induced leukocyte transmigration was unaltered in the KO mice. WT mice treated with the anti-ICAM-2 mAb also exhibited a selective reduction in leukocyte transmigration in response to IL-1 beta while an anti-ICAM-1 mAb inhibited both leukocyte adhesion and transmigration. Interestingly, mAb 3C4 significantly suppressed IL-1 beta induced neutrophil transmigration in PECAM-1 KO animals in the peritonitis model but not in the cremaster muscle. The findings provide direct evidence for the involvement of ICAM-2 in neutrophil transmigration in vivo, though this role appears to be stimulus specific. Furthermore, ICAM-2 appears capable of mediating PECAM-1-independent leukocyte transmigration.
机构:
Univ London Imperial Coll Sci & Technol, Eric Bywaters Ctr Vasc Inflammat, Cardiovasc Med Unit, Hammersmith Hosp,Fac Med, London W12 0NN, EnglandUniv London Imperial Coll Sci & Technol, Eric Bywaters Ctr Vasc Inflammat, Cardiovasc Med Unit, Hammersmith Hosp,Fac Med, London W12 0NN, England
Dangerfield, JP
Wang, SJ
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Univ London Imperial Coll Sci & Technol, Eric Bywaters Ctr Vasc Inflammat, Cardiovasc Med Unit, Hammersmith Hosp,Fac Med, London W12 0NN, EnglandUniv London Imperial Coll Sci & Technol, Eric Bywaters Ctr Vasc Inflammat, Cardiovasc Med Unit, Hammersmith Hosp,Fac Med, London W12 0NN, England
Wang, SJ
Nourshargh, S
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Univ London Imperial Coll Sci & Technol, Eric Bywaters Ctr Vasc Inflammat, Cardiovasc Med Unit, Hammersmith Hosp,Fac Med, London W12 0NN, EnglandUniv London Imperial Coll Sci & Technol, Eric Bywaters Ctr Vasc Inflammat, Cardiovasc Med Unit, Hammersmith Hosp,Fac Med, London W12 0NN, England
机构:
Univ London Imperial Coll Sci & Technol, Eric Bywaters Ctr Vasc Inflammat, Cardiovasc Med Unit, Hammersmith Hosp,Fac Med, London W12 0NN, EnglandUniv London Imperial Coll Sci & Technol, Eric Bywaters Ctr Vasc Inflammat, Cardiovasc Med Unit, Hammersmith Hosp,Fac Med, London W12 0NN, England
Dangerfield, JP
Wang, SJ
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机构:
Univ London Imperial Coll Sci & Technol, Eric Bywaters Ctr Vasc Inflammat, Cardiovasc Med Unit, Hammersmith Hosp,Fac Med, London W12 0NN, EnglandUniv London Imperial Coll Sci & Technol, Eric Bywaters Ctr Vasc Inflammat, Cardiovasc Med Unit, Hammersmith Hosp,Fac Med, London W12 0NN, England
Wang, SJ
Nourshargh, S
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机构:
Univ London Imperial Coll Sci & Technol, Eric Bywaters Ctr Vasc Inflammat, Cardiovasc Med Unit, Hammersmith Hosp,Fac Med, London W12 0NN, EnglandUniv London Imperial Coll Sci & Technol, Eric Bywaters Ctr Vasc Inflammat, Cardiovasc Med Unit, Hammersmith Hosp,Fac Med, London W12 0NN, England