Localization of oestrogen receptor α, oestrogen receptor β and androgen receptors in the rat reproductive organs

被引:358
作者
Pelletier, G [1 ]
Labrie, C [1 ]
Labrie, F [1 ]
机构
[1] Univ Laval, CHU Laval, Ctr Rech, Mol Endocrinol Lab, Quebec City, PQ G1V 4G2, Canada
关键词
D O I
10.1677/joe.0.1650359
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
There is now evidence that oestrogens and androgens can influence male and female reproductive systems. In order to accurately identify the sites of action of oestrogens and androgens, we have proceeded to the histological localization of the two oestrogen receptor (ER) subtypes, ER alpha and ER beta, and the androgen receptor (AR) in the reproductive tissues of adult rats of both sexes. AR was detected by immunocytochemistry, while ER alpha and ER beta were localized by both immunocytochemistry and in situ hybridization. In the pituitary gland of animals of both sexes, ER alpha was found in the majority of nuclei of secretory cells in the anterior pituitary. The intermediate and posterior lobes did not show any staining. ER beta was not found to be expressed in any of the pituitary lobes. Using AR antibodies, nuclear staining was detected in about 50% of secretory cells of the anterior lobe, the intermediate and posterior lobes being completely unstained. In the testis, ER alpha was localized in nuclei of Leydig cells as well as in round spermatocytes and spermatids, while ER beta could only be detected in Sertoli cell nuclei. AR immunoreactivity was found in nuclei of Sertoli, peritubular myoid and Leydig cells. In the prostate, ER beta was observed in epithelial cells of tubulo-alveoli, while the stroma was unlabelled. ER alpha was not found to be expressed in any prostate cells. In the prostate, AR was detected in nuclei of epithelial, stromal and endothelial cells. In seminal vesicles, staining of ER alpha was found in nuclei of epithelial and stromal cells. Similar findings were observed using AR antibodies. While ER beta mRNA could not be detected by in situ hybridization, weak staining for ER beta was localized in epithelial cells of seminal vesicles. In the ovary, both ER alpha and ERP were found to be expressed. ER beta mRNA was found in granulosa cells of growing follicles, while ER alpha was present in theca cells, interstitial gland cells and germinal epithelium. AR immunoreactivity was detected in granulosa cell nuclei in growing follicles and also in scattered interstitial cells. In the oviduct and uterus, ER alpha was observed in nuclei of epithelial cells as well as of stromal and muscle cells. Similarly, AR immunoreactivity was present in nuclei of epithelial cells, stromal and muscle cells in both the oviduct and uterus. ER beta was not detected in the oviduct and uterus. The present findings indicate a cell-specific localization of ER alpha, ER beta and AR in reproductive tissues in rats of both sexes. By establishing the precise sites of action of oestrogens and androgens they contribute to a better understanding of the respective role of these steroids in reproduction function.
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页码:359 / 370
页数:12
相关论文
共 35 条
  • [1] ARMSTRONG DT, 1976, ENDOCRINOLOGY, V99, P987
  • [2] Estrogen receptor-beta mRNA expression in rat ovary: Down-regulation by gonadotropins
    Byers, M
    Kuiper, GGJM
    Gustafsson, JA
    ParkSarge, OK
    [J]. MOLECULAR ENDOCRINOLOGY, 1997, 11 (02) : 172 - 182
  • [3] STEROID-RECEPTOR FAMILY - STRUCTURE AND FUNCTIONS
    CARSONJURICA, MA
    SCHRADER, WT
    OMALLEY, BW
    [J]. ENDOCRINE REVIEWS, 1990, 11 (02) : 201 - 220
  • [4] Clark JH, 1992, TXB ENDOCRINOLOGY, P35
  • [5] ANDROGEN RECEPTOR EXPRESSION IN HUMAN TISSUES - AN IMMUNOHISTOCHEMICAL STUDY
    DEWINTER, JAR
    TRAPMAN, J
    VERMEY, M
    MULDER, E
    ZEGERS, ND
    VANDERKWAST, TH
    [J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1991, 39 (07) : 927 - 936
  • [6] Localization of type 5 17β-hydroxysteroid dehydrogenase, 3β-hydroxysteroid dehydrogenase, and androgen receptor in the human prostate by in situ hybridization and immunocytochemistry
    El-Alfy, M
    Luu-The, V
    Huang, XF
    Berger, L
    Labrie, F
    Pelletier, G
    [J]. ENDOCRINOLOGY, 1999, 140 (03) : 1481 - 1491
  • [7] Human estrogen receptor β-gene structure, chromosomal localization, and expression pattern
    Enmark, E
    Pelto-Huikko, M
    Grandien, K
    Lagercrantz, S
    Lagercrantz, J
    Fried, G
    Nordenskjöld, M
    Gustafsson, JÅ
    [J]. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1997, 82 (12) : 4258 - 4265
  • [8] THE STEROID AND THYROID-HORMONE RECEPTOR SUPERFAMILY
    EVANS, RM
    [J]. SCIENCE, 1988, 240 (4854) : 889 - 895
  • [9] Characterization of mice deficient in aromatase (ArKO) because of targeted disruption of the cyp19 gene
    Fisher, CR
    Graves, KH
    Parlow, AF
    Simpson, ER
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (12) : 6965 - 6970
  • [10] Expression of estrogen receptor-β protein in rodent ovary
    Fitzpatrick, SL
    Funkhouser, JM
    Sindoni, DM
    Stevis, PE
    Deecher, DC
    Bapat, AR
    Merchenthaler, I
    Frail, DE
    [J]. ENDOCRINOLOGY, 1999, 140 (06) : 2581 - 2591