Two approaches for irradiating cells individually: a charged-particle microbeam and a soft X-ray microprobe

被引：25

作者：

Folkard, M ^{[1
]}

Vojnovic, B ^{[1
]}

Schettino, G ^{[1
]}

Forsberg, M ^{[1
]}

Bowey, G ^{[1
]}

Prise, KM ^{[1
]}

Michael, BD ^{[1
]}

Michette, AG ^{[1
]}

Pfauntsch, SJ ^{[1
]}

机构：

[1] UNIV LONDON KINGS COLL,LONDON WC2R 2LS,ENGLAND

来源：

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS | 1997年 / 130卷 / 1-4期

关键词：

D O I：

10.1016/S0168-583X(97)00276-0

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

We are developing two independent, but complementary microbeams for irradiating cells individually in vitro. Firstly, a charged-particle microbeam that uses a fine-bore glass capillary, combined with a transmission detector to precisely irradiate cells with exact numbers of energetic charge-particles and secondly, a soft X-ray microprobe that produces a very fine beam of carbon-K (278 eV) ultrasoft X-rays, focused to a spot size < 100 nm. X-ray focusing is achieved using zone-plate diffraction lenses developed initially for X-ray microscopy applications.

引用

页码：270 / 274

页数：5

共 7 条

[1] Cole A, 1980, RAD BIOL CANCER RES, P33
[2] Alpha-particle-induced sister chromatid exchange in normal human lung fibroblasts: Evidence for an extranuclear target
Deshpande, A
Goodwin, EH
Bailey, SM
Marrone, BL
Lehnert, BE
[J]. RADIATION RESEARCH, 1996, 145 (03) : 260 - 267
[3] FOLKARD M, 1995, RADIAT PROT DOSIM, V65, P215
[4] MICHETTE AG, 1992, XRAY MICROSCOPY, V3
[5] Clastogenic effects of defined numbers of 3.2 MeV alpha particles on individual CHO-K1 cells
Nelson, JM
Brooks, AL
Metting, NF
Khan, MA
Buschbom, RL
Duncan, A
Miick, R
Braby, LA
[J]. RADIATION RESEARCH, 1996, 145 (05) : 568 - 574
[6] RADIOBIOLOGY OF ALPHA-PARTICLES .3. CELL INACTIVATION BY ALPHA-PARTICLE TRAVERSALS OF THE CELL-NUCLEUS
RAJU, MR
EISEN, Y
CARPENTER, S
INKRET, WC
[J]. RADIATION RESEARCH, 1991, 128 (02) : 204 - 209
[7] IRRADIATION OF PARTS OF INDIVIDUAL CELLS
ZIRKLE, RE
BLOOM, W
[J]. SCIENCE, 1953, 117 (3045) : 487 - 493

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