Factor Xa induces mitogenesis of coronary artery smooth muscle cell via activation of PAR-2

被引：48

作者：

Koo, BH

Chung, KH

Hwang, KC

Kim, DS ^{[1
]}

机构：

[1] Yonsei Univ, Dept Biochem, Coll Sci, Seoul 120749, South Korea

[2] Yonsei Univ, Bioprod Res Ctr, Seoul 120749, South Korea

[3] Yonsei Univ, Coll Med, Inst Cardiovasc Res, Seoul 120752, South Korea

来源：

FEBS LETTERS | 2002年 / 523卷 / 1-3期

关键词：

coronary artery smooth muscle cell; factor Xa; protease-activated receptor-2;

D O I：

10.1016/S0014-5793(02)02948-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Factor Xa-induced stimulation of coronary artery smooth muscle cells (CASMC) was investigated by analyzing [H-3]thymidine incorporation, cell proliferation, and ERK-1/2 activation. Exposure of the cells to factor Xa evoked a time-dependent activation of ERK-1/2 with increased [H-3]thymidine incorporation and cell proliferation. The factor Xa-induced ERK-1/2 activation was not desensitized by preincubation of the cells with thrombin. However, ERK-1/2 activation was markedly attenuated by prior exposure of the cells to protease-activated receptor-2 (PAR-2) activating peptide, SLIGKV. The mitogenic effect of factor Xa was significantly reduced in the presence of anti-PAR-2 monoclonal antibody. Several lines of experimental evidence indicate that factor Xa-induced mitogenesis of CASMC is a cellular process mediated by PAR-2 activation. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：85 / 89

页数：5

共 48 条

[1] Mast cell tryptase stimulates human lung fibroblast proliferation via protease-activated receptor-2 [J].