The genome and transcriptomes of the anti-tumor agent Clostridium novyi-NT

被引：116

作者：

Bettegowda, Chetan

Huang, Xin

Lin, Jimmy

Cheong, Ian

Kohli, Manu

Szabo, Stephen A.

Zhang, Xiaosong

Diaz, Luis A., Jr.

Velculescu, Victor E.

Parmigiani, Giovanni

Kinzler, Kenneth W.

Vogelstein, Bert

Zhou, Shibin

机构：

[1] Johns Hopkins Med Inst, Howard Hughes Med Inst, Ludwig Ctr Canc Genet & Therapeut, Sidney Kimmel Comprehens Canc Ctr, Baltimore, MD 21231 USA

[2] Johns Hopkins Med Inst, Dept Pharmacol & Mol Sci, Baltimore, MD 21231 USA

[3] Johns Hopkins Med Inst, Dept Oncol, Baltimore, MD 21205 USA

[4] Johns Hopkins Med Inst, Dept Biostat, Baltimore, MD 21205 USA

[5] Johns Hopkins Med Inst, Dept Pathol, Baltimore, MD 21205 USA

来源：

NATURE BIOTECHNOLOGY | 2006年 / 24卷 / 12期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1038/nbt1256

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bacteriolytic anti-cancer therapies employ attenuated bacterial strains that selectively proliferate within tumors. Clostridium novyi-NT spores represent one of the most promising of these agents, as they generate potent anti-tumor effects in experimental animals. We have determined the 2.55-Mb genomic sequence of C. novyi-NT, identifying a new type of transposition and 139 genes that do not have homologs in other bacteria. The genomic sequence was used to facilitate the detection of transcripts expressed at various stages of the life cycle of this bacterium in vitro as well as in infections of tumors in vivo. Through this analysis, we found that C. novyi-NT spores contained mRNA and that the spore transcripts were distinct from those in vegetative forms of the bacterium.

引用

页码：1573 / 1580

页数：8

共 50 条

[1] Bacteriolytic therapy can generate a potent immune response against experimental tumors [J].

Agrawal, N ;

Bettegowda, C ;

Cheong, I ;

Geschwind, JF ;

Drake, CG ;

Hipkiss, EL ;

Tatsumi, M ;

Dang, LH ;

Diaz, LA ;

Pomper, M ;

Abusedera, M ;

Wahl, RL ;

Kinzler, KW ;

Zhou, SB ;

Huso, DL ;

Vogelstein, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (42) :15172-15177

[2] Transcriptional program of early sporulation and stationary-phase events in Clostridium acetobutylicum [J].

Alsaker, KV ;

Papoutsakis, ET .

JOURNAL OF BACTERIOLOGY, 2005, 187 (20) :7103-7118

[3]

Arthur JR, 2000, CELL MOL LIFE SCI, V57, P1825

[4] The use of clostridial spores for cancer treatment [J].

Barbe, S. ;

Van Mellaert, L. ;

Anne, J. .

JOURNAL OF APPLIED MICROBIOLOGY, 2006, 101 (03) :571-578

[5] Overcoming the hypoxic barrier to radiation therapy with anaerobic bacteria [J].

Bettegowda, C ;

Dang, LH ;

Abrams, R ;

Huson, DL ;

Dillehay, L ;

Cheong, I ;

Agrawal, N ;

Borzillary, S ;

McCaffery, JM ;

Watson, EL ;

Lin, KS ;

Bunz, F ;

Baidoo, K ;

Pomper, MG ;

Kinzler, KW ;

Vogelstein, B ;

Zhou, SB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (25) :15083-15088

[6] The genome sequence of Clostridium tetani, the causative agent of tetanus disease [J].

Brüggemann, H ;

Bäumer, S ;

Fricke, WF ;

Wiezer, A ;

Liesegang, H ;

Decker, I ;

Herzberg, C ;

Martínez-Arias, R ;

Merkl, R ;

Henne, A ;

Gottschalk, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (03) :1316-1321

[7] REVIVAL AND IDENTIFICATION OF BACTERIAL-SPORES IN 25-MILLION-YEAR-OLD TO 40-MILLION-YEAR-OLD DOMINICAN AMBER [J].

CANO, RJ ;

BORUCKI, MK .

SCIENCE, 1995, 268 (5213) :1060-1064

[8] PCR-Assisted Contig extension: Stepwise strategy for bacterial genome closure [J].

Carraro, DM ;

Camargo, AA ;

Salim, ACM ;

Grivet, M ;

Vasconcelos, AT ;

Simpson, AJG .

BIOTECHNIQUES, 2003, 34 (03) :626-+

[9] Colorectal carcinoma in endoscopic biopsies; additional histologic criteria for the diagnosis [J].

Cerar, A ;

Zidar, N ;

Vodopivec, B .

PATHOLOGY RESEARCH AND PRACTICE, 2004, 200 (10) :657-662

[10]

CHAMBON P, 1968, J BIOL CHEM, V243, P5110

← 1 2 3 4 5 →