Chemo-enzymatic synthesis of fluorinated 2-N-acetamidosugar nucleotides using UDP-GlcNAc pyrophosphorylase

被引：24

作者：

Feng, F

Okuyama, K

Niikura, K

Ohta, T

Sadamoto, R

Monde, K

Noguchi, T

Nishimura, SI ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Sci, Div Biol Sci, Lab Biomacromol Chem, Sapporo, Hokkaido 0010021, Japan

[2] YAMASA Corp, Div Biochem, Bioprod Lab, Chiba 2880056, Japan

[3] Natl Inst Adv Ind Sci & Technol, Sapporo, Hokkaido 0628517, Japan

[4] Hokkaido Univ, Grad Sch Sci, Div Biol Sci, Shionogi Lab Biomol Chem, Sapporo, Hokkaido 0010021, Japan

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2004年 / 2卷 / 11期

关键词：

D O I：

10.1039/b402860k

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Two non-natural. uorinated 2-N-acetamidosugar nucleotides, uridine 5'-diphosphate (UDP) 2-acetamido-2,4-dideoxy-4-fluoro-alpha-D-glucopyranose (UDP-4-FGlcNAc) 1 and its galacto isomer (UDP-4-FGalNAc) 2, were enzymatically constructed by treating chemically synthesized. uorinated 2-N-acetamidosugar 1-phosphates as the donor with UDP 2-acetamido-2-deoxy-alpha-D-glucopyranose pyrophosphorylase in the presence of uridine 5'-triphosphate (UTP).

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页码：1617 / 1623

页数：7

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