Enhanced 2,3-butanediol production by Klebsiella oxytoca using a two-stage agitation speed control strategy

被引：130

作者：

Ji, Xiao-Jun ^{[1
]}

Huang, He ^{[1
]}

Du, Jun ^{[1
]}

Zhu, Jian-Guo ^{[1
]}

Ren, Lu-Jing ^{[1
]}

Hu, Nan ^{[1
]}

Li, Shuang ^{[1
]}

机构：

[1] Nanjing Univ Technol, State Key Lab Mat Oriented Chem Engn, Coll Life Sci & Pharm, Nanjing 210009, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2009年 / 100卷 / 13期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

2,3-Butanediol; Klebsiella oxytoca; Batch fermentation; Two-stage control strategy; ENTEROBACTER-AEROGENES; GLUCOSE; PNEUMONIAE; FERMENTATION; OPTIMIZATION; BIOREACTOR; AERATION; XYLOSE;

D O I：

10.1016/j.biortech.2009.02.031

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Batch fermentative production of 2,3-butanediol by Klebsiella oxytoca was investigated using various oxygen supply methods though varying agitation speed. Based on the analysis of three kinetic parameters including specific cell growth rate (mu). specific glucose consumption rate (q(s)) and specific 2,3-butanediol formation rate (q(p)), a two-stage agitation speed control strategy, aimed at achieving high concentration, high yield and high productivity of 2.3-butanediol, was proposed. At the first 15 h, agitation speed was controlled at 300 rpm to obtain high mu for cell growth, subsequently agitation speed was controlled at 200 rpm to maintain high q(p) for high 2.3-butanediol accumulation. Finally, the maximum concentration of 2,3-butanediol reached 95.5 g l(-1) with the yield of 0.478 g g(-1) and the productivity of 1.71 g l(-1) h(-1), which were 6.23%, 6.22% and 22.14% over the best results controlled by constant agitation speeds. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved.

引用

页码：3410 / 3414

页数：5

共 25 条

[1] OPTIMIZATION OF 2,3-BUTANEDIOL PRODUCTION BY KLEBSIELLA-OXYTOCA THROUGH OXYGEN-TRANSFER RATE CONTROL [J].