SADB phosphorylation of γ-tubulin regulates centrosome duplication

被引：69

作者：

Alvarado-Kristensson, Maria ^{[1
,3
]}

Josefa Rodriguez, Maria ^{[2
]}

Silio, Virginia ^{[1
]}

Valpuesta, Jose M. ^{[2
]}

Carrera, Ana C. ^{[1
]}

机构：

[1] CSIC, Ctr Nacl Biotecnol, Dept Immunol & Oncol, E-28049 Madrid, Spain

[2] CSIC, Ctr Nacl Biotecnol, Dept Macromol Struct, E-28049 Madrid, Spain

[3] Lund Univ, Malmoe Univ Hosp, Dept Lab Med, Ctr Mol Pathol, S-22100 Lund, Sweden

来源：

NATURE CELL BIOLOGY | 2009年 / 11卷 / 09期

关键词：

MICROTUBULE NUCLEATION; CELL-CYCLE; CENTRIOLE DUPLICATION; TRANSCRIPTION FACTORS; GENE-EXPRESSION; BUDDING YEAST; RING COMPLEX; PROTEIN; KINASE; RECRUITMENT;

D O I：

10.1038/ncb1921

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Symmetrical cell division requires duplication of DNA and protein content to generate two daughter cells. Centrosomes also duplicate during cell division, but the mechanism controlling this process is incompletely understood. We describe an alternative splice form of SadB encoding a short SADB Ser/Thr kinase whose activity fluctuates during the cell cycle, localizes to centrosomes, and controls centrosome duplication. Reduction of endogenous SADB levels diminished centrosome numbers, whereas enhanced SADB expression induced centrosome amplification. SADB exerted this action through phosphorylation of gamma-tubulin on Ser 131, as expression of a phosphomimetic Ser 131-to-Asp gamma-tubulin mutant alone increased centrosome numbers, whereas non-phosphorylatable Ala 131-gamma-tubulin impaired centrosome duplication. We propose that SADB kinase activity controls centrosome homeostasis by regulating phosphorylation of gamma-tubulin.

引用

页码：1081 / U86

页数：17

共 41 条

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