Molecular cytogenetic analysis of Leymus racemosus chromosomes added to wheat

被引：97

作者：

Qi, LL

Wang, SL

Chen, PD

Liu, DJ

Friebe, B

Gill, BS

机构：

[1] KANSAS STATE UNIV, DEPT PLANT PATHOL, MANHATTAN, KS 66506 USA

[2] NANGJING AGR UNIV, DEPT AGRON, Nanjing 210095, PEOPLES R CHINA

来源：

THEORETICAL AND APPLIED GENETICS | 1997年 / 95卷 / 07期

关键词：

Triticum aestivum; Leymus racemosus; chromosome addition lines; C-banding; RLFP analysis; homoeology;

D O I：

10.1007/s001220050666

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 [作物学];

摘要：

Five disomic, two double-disomic, and two ditelosomic addition lines and one disomic substitution line derived from the crosses of Triticum aestivum (2n = 6x = 42, AABBDD) x Leymus racemosus (2n = 4x; = 28, JJNN) were identified by C-banding analysis, The homoeology of the added Leymus chromosomes was determined by RFLP analysis. Four of five disomic addition lines belonged to group 2, 5, 6 and 7 chromosomes of L. racemosus; these were designated as 2Lr#1 (NAU516), 5Lr#1 (NAU504, NAU514), 6Lr#1 (NAU512), and 7Lr#1 (NAU501). Two additional chromosomes, 1Lr#1 and 3Lr#1, were present in double-disomic addition lines 1Lr#1 + 5Lr#1 (NAU525) and 3Lr#1 + 7Lr#1 (NAU524), respectively. In the disomic substitution line wheat chromosome 2B was replaced by L. racemosus chromosome 2Lr#1 (NAU551), Two telocentric chromosomes, 2Lr#2S (NAU509) and 7Lr#1S (NAU511), were isolated as dilelosomic addition lines. The study presented here provides the first evidence of homoeology of the added L. racemosus chromosomes with wheat chromosomes using DNA markers. Our data provide the basis for further directed chromosome engineering aimed at producing compensating wheat-L. racemosus translocation lines.

引用

页码：1084 / 1091

页数：8

共 33 条

[1]

CHEN PD, 1993, P 8 INT WHEAT GEN S, P153

[2]

IDENTIFICATION OF WHEAT AGROPYRON-CRISTATUM MONOSOMIC ADDITION LINES BY RFLP ANALYSIS USING A SET OF ASSIGNED WHEAT DNA PROBES [J].