AS-IV protects against kidney IRI through inhibition of NF-κB activity and PUMA upregulation

被引:13
作者
Xin, Yan [1 ]
Li, Gang [2 ]
Liu, Hongxiu [2 ]
Ai, Dengbin [1 ]
机构
[1] Qingdao Municipal Hosp, Dept Anesthesia, Qingdao, Shandong, Peoples R China
[2] Peoples Hosp Rizhao, Dept Anesthesia, Rizhao, Shandong, Peoples R China
关键词
Kidney; ischemia reperfusion injury (IRI); NF-kappa B; p53 upregulated modulator of apoptosis (PUMA); apoptosis; inflammatory genes; astragaloside IV (AS-IV); RENAL ISCHEMIA/REPERFUSION INJURY; ASTRAGALOSIDE-IV; ISCHEMIA; APOPTOSIS; INFLAMMATION; CONTRIBUTES; DEATH;
D O I
暂无
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
100103 [病原生物学]; 100218 [急诊医学];
摘要
Objective: To determine and explore the effect of Astragalus saponin IV (AS-IV) on ischemia/reperfusion (IR)-induced renal injury and its mechanisms. Methods: Experimental model of renal I/R was induced in rats by bilateral renal artery clamp for 45 min followed by reperfusion of 6 h. Rats were divided into three groups: (1) sham (2) IRI (3) IRI/AS-IV. In IRI/AS-IV groups, AS-IV was orally administered once a day to rats at 2 mg . kg(-1) . d(-1) for 7 days prior to ischemia. At 6 h after reperfusion, the inflammatory cytokines and renal function was assessed and NF-kappa B activity and PUMA expression was detected. Apoptotic cells was detected by TUNEL assay. Results: AS-IV significantly decreased serum and tissue levels of IL-6 and TNF-alpha, and reduced apoptotic cell counts and histological damage. AS-IV down-regulated the phosphorylation of p65 subunit of NF-kappa B (NF-kappa B p65) and PUMA expression, and the NF-kappa B activity compared to the I/R groups. Conclusions: AS-IV provided protection against IRI-induced renal injury by reducing apoptosis and inflammation through inhibition of NF-kappa B activity and PUMA expression. AS-IV pre-treatment ameliorated tubular damage and suppressed the NF-kappa B p65 expression.
引用
收藏
页码:18293 / 18301
页数:9
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