Effects of Naringin on the Proliferation, Migration and Osteogenesis of Two Human Osteoblast-Like Cell Lines

被引:6
作者
Guo, Li-Hua [1 ,2 ]
Cao, Yu [3 ]
Zhuang, Run-Tao [4 ]
Wang, Ling-Xiao [1 ]
Han, Yan [2 ]
Li, Jun [1 ]
机构
[1] Capital Med Univ, Beijing Stomatol Hosp, Dept Dent Implant Ctr, Sch Stomatol, Beijing 100050, Peoples R China
[2] China Acad Chinese Med Sci, Dept Stomatol, Xiyuan Hosp, Beijing 100091, Peoples R China
[3] Capital Med Univ, Beijing Stomatol Hosp, Sch Stomatol, Dept Integrated Emergency Dent Care, Beijing 100050, Peoples R China
[4] Beijing Jiaotong Univ, Dept Stomatol, Community Hlth Ctr, Beijing 100044, Peoples R China
基金
中国国家自然科学基金;
关键词
Naringin; Proliferation; Migration; Osteogenesis; Hedgehog Signaling Pathway; DIABETES-MELLITUS; BONE-FORMATION; ORAL IMPLANTS; GROWTH-FACTOR; STEM-CELLS; DIFFERENTIATION; OSSEOINTEGRATION; EXPRESSION; SURVIVAL; RHBMP-2;
D O I
10.1166/jbt.2018.1811
中图分类号
Q813 [细胞工程];
学科分类号
100113 [医学细胞生物学];
摘要
This study aimed to investigate the effects of naringin on osteoblast-like cell proliferation, migration and osteogenesis and GLI1 expression in vitro. CCK-8 assays and flow cytometry analysis were used to evaluate the effects of naringin on MG-63 and U-20S cell proliferation. Transwell migration assays were performed to detect cell migration. Alkaline phosphatase activity, Alizarin Red S staining and osteogenic genes (BMP2, RUNX2, COL-1) were assessed to determine the cell osteogenic ability. GLI1 mRNA and protein levels were measured by qRT-PCR and Western blot analyses. The results showed that naringin at a dose of 1 x 10(-5) mol/l for MG-63 cells and 1 x 10(-8) mol/l for U-20S cells enhanced the cell proliferation, migration and osteogenesis compared with those of the control group (P < 0.05). In addition, GLI1 expression was significantly increased compared to that of the control. These results indicated that naringin promotes proliferation, migration and osteogenesis of human osteoblast-like cells. Additionally, naringin activated the Hedgehog signaling pathway. Our findings provide insights into the mechanistic link between naringin-induced osteogenesis and the Hedgehog signaling pathway.
引用
收藏
页码:871 / 879
页数:9
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