Insight into the molecular evolution of two tropinone reductases

被引：20

作者：

Nakajima, K ^{[1
]}

Oshita, Y ^{[1
]}

Yamada, Y ^{[1
]}

Hashimoto, T ^{[1
]}

机构：

[1] Nara Inst Sci & Technol, Grad Sch Biol Sci, Ikoma, Nara 6300101, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1999年 / 63卷 / 10期

关键词：

molecular evolution; short-chain dehydrogenase/reductase; substrate specificity; tropane alkaloids; tropinone reductase;

D O I：

10.1271/bbb.63.1819

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In tropane alkaloid biosynthesis, two tropinone reductases produce different stereoisomers from a common substrate, tropinone. The two enzymes share 64% of identical amino acids, and highly homologous proteins with variable substrate-binding residues have also been found in tropane alkaloid non-producing species. This exemplifies a simple evolutionary process that plants have taken to acquire a new secondary metabolic pathway.

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收藏

页码：1819 / 1822

页数：4

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