Molecular cloning, functional analysis, and RNA expression analysis of connexin45.6: a zebrafish cardiovascular connexin

被引:13
作者
Christie, TL
Mui, R
White, TW
Valdimarsson, G [1 ]
机构
[1] Univ Manitoba, Dept Zool, Winnipeg, MB R3T 2N2, Canada
[2] SUNY Stony Brook, Dept Physiol & Biophys, Stony Brook, NY 11794 USA
[3] Univ Manitoba, Dept Biochem & Med Genet, Winnipeg, MB R3E 0W3, Canada
来源
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 2004年 / 286卷 / 05期
关键词
gap junction; Danio rerio; cardiovascular;
D O I
10.1152/ajpheart.00800.2003
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
In the vertebrate cardiovascular system, gap junctions function in intercellular communication essential for both the coordinated propagation of the heartbeat and the control of vasomotor responses in the vascular system. Connexins, the protein subunits of gap junctions, are coded by a multigene family. In this study, a connexin gene (zfCx45.6), which exhibits 53% amino acid identity to chick Cx42, was cloned from zebrafish genomic DNA. With the use of the LN54 radiation hybrid panel, zfCx45.6 was mapped to zebrafish linkage group 9. Northern blots and RT-PCR revealed the presence of zfCx45.6 mRNA in the embryo before 2 h postfertilization (hpf) and then again beginning at about 12 hpf, after which time no major changes in relative expression levels were detected. In the adult, zfCx45.6 mRNA continued to be detected in the heart, as well as the brain, liver, and ovary, but not the lens. Whole mount in situ hybridization revealed zfCx45.6 mRNA was expressed at high levels in the major vessels of the entire embryo and in both the atrium and ventricle of the adult heart. Expression of zfCx45.6 channels in paired Xenopus oocytes produced high levels of intercellular coupling that was voltage sensitive. With the previous isolation of zebrafish Cx43 and Cx43.4, zebrafish orthologues have now been isolated for three of the four connexins expressed in the mammalian cardiovascular system.
引用
收藏
页码:H1623 / H1632
页数:10
相关论文
共 70 条
[1]  
Amemiya CT, 1999, METHOD CELL BIOL, V60, P235
[2]   Generation of a zebrafish P1 artificial chromosome library [J].
Amemiya, CT ;
Zon, LI .
GENOMICS, 1999, 58 (02) :211-213
[3]   Species-specific voltage-gating properties of connexin-45 junctions expressed in Xenopus oocytes [J].
Barrio, LC ;
Capel, J ;
Jarillo, JA ;
Castro, C ;
Revilla, A .
BIOPHYSICAL JOURNAL, 1997, 73 (02) :757-769
[4]   GAP-JUNCTIONS FORMED BY CONNEXIN-26 AND CONNEXIN-32 ALONE AND IN COMBINATION ARE DIFFERENTLY AFFECTED BY APPLIED VOLTAGE [J].
BARRIO, LC ;
SUCHYNA, T ;
BARGIELLO, T ;
XU, LX ;
ROGINSKI, RS ;
BENNETT, MVL ;
NICHOLSON, BJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (19) :8410-8414
[5]   Changing patterns of ganglion cell coupling and connexin expression during chick retinal development [J].
Becker, DL ;
Bonness, V ;
Catsicas, M ;
Mobbs, P .
JOURNAL OF NEUROBIOLOGY, 2002, 52 (04) :280-293
[6]   Expression of major gap junction connexin types in the working myocardium of eight chordates [J].
Becker, DL ;
Cook, JE ;
Davies, CS ;
Evans, WH ;
Gourdie, RG .
CELL BIOLOGY INTERNATIONAL, 1998, 22 (7-8) :527-543
[7]  
BEYER EC, 1990, J BIOL CHEM, V265, P14439
[8]   CONNEXIN43 - A PROTEIN FROM RAT-HEART HOMOLOGOUS TO A GAP JUNCTION PROTEIN FROM LIVER [J].
BEYER, EC ;
PAUL, DL ;
GOODENOUGH, DA .
JOURNAL OF CELL BIOLOGY, 1987, 105 (06) :2621-2629
[9]   CONNEXIN40, A COMPONENT OF GAP-JUNCTIONS IN VASCULAR ENDOTHELIUM, IS RESTRICTED IN ITS ABILITY TO INTERACT WITH OTHER CONNEXINS [J].
BRUZZONE, R ;
HAEFLIGER, JA ;
GIMLICH, RL ;
PAUL, DL .
MOLECULAR BIOLOGY OF THE CELL, 1993, 4 (01) :7-20
[10]   Connections with connexins: The molecular basis of direct intercellular signaling [J].
Bruzzone, R ;
White, TW ;
Paul, DL .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1996, 238 (01) :1-27