A direct enzymatic synthesis of beta-D-galactopyranosyl-D-xylopyranosides and their use to evaluate rat intestinal lactase activity in vivo

被引：12

作者：

Aragon, JJ

Canada, FJ

FernandezMayoralas, A

Lopez, R

MartinLomas, M

Villanueva, D

机构：

[1] CSIC,INST QUIM ORGAN,GRP CARBOHIDRATOS,E-28006 MADRID,SPAIN

[2] UNIV AUTONOMA MADRID,FAC MED,DEPT BIOQUIM,E-28029 MADRID,SPAIN

来源：

CARBOHYDRATE RESEARCH | 1996年 / 290卷 / 02期

关键词：

intestinal lactase evaluation; beta-D-galactopyranosyl-D-xylopyranosides; enzymatic glycosylation; E-coli beta-D-galactosidase;

D O I：

10.1016/0008-6215(96)00113-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By enzymatic beta-D-galactosylation of D-xylose a mixture of 4-, 3-, and 2-O-beta-D-galactopyranosyl-D-xyloses (1, 4, and 7, respectively) was obtained in 50% isolated yield, Disaccharides 1, 4, and 7 are substrates of intestinal lactase isolated from lamb small intestine with K-m values of 250.0, 4.5, and 14.0 mM, respectively, The mixture was used to monitor the normal decline in lactase activity in rats that takes place after weaning. The data obtained by this method correlated with the levels of intestinal lactase activity in the same animals after being sacrificed. (C) 1996 Elsevier Science Ltd.

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收藏

页码：209 / 216

页数：8

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