Glial growth factor 2 induces proliferation and structural changes in ensheathing cells

被引:30
作者
Chuah, MI
Cossins, JM
Woodhall, E
Tennent, R
Nash, G
West, AK
机构
[1] Univ Tasmania, Dept Anat & Physiol, Hobart, Tas 7001, Australia
[2] Univ Tasmania, Dept Biochem, Hobart, Tas 7001, Australia
[3] Australian Antarctic Div, Electron Microscopy Unit, Kingston, Tas 7050, Australia
基金
澳大利亚研究理事会;
关键词
ensheathing cell; glial growth factor 2; ultrastructure; proliferation; tissue culture; neuregulin;
D O I
10.1016/S0006-8993(99)02455-5
中图分类号
Q189 [神经科学];
学科分类号
071006 [神经生物学];
摘要
Ensheathing cells were isolated from neonatal rat olfactory bulbs and cultured in the presence of glial growth factor 2 (GGF2). Proliferation assay showed that at concentrations of up to 60 ng/ml GGF2, ensheathing cells underwent a modest increase in proliferation rate. This stimulation was not maintained at high doses of GGF2 at 100 ng/ml or more. Chemotaxis chambers and scanning electron microscopy were used to determine whether GGF2 was a chemoattractant for ensheathing cells. Although the results showed no chemotactic response to GGF2, ensheathing cells demonstrated structural changes when cultured in the presence of 20 ng/ml GGF2. Ultrastructural observations revealed that GGF2 promoted increased deposition of extracellular matrix on the cell membrane, more cytoskeletal elements in the processes and as a possible consequence, contributed to a more rigid support. Ensheathing cells cultured in the absence of GGF2 often extended thinner and curved processes. Reverse transcription-polymerase chain reaction confirmed the presence of GGF2 transcripts in ensheathing cells, suggesting that ensheathing cells themselves are a source of GGF2. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:265 / 274
页数:10
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