Physical map location of the multicopy genes coding for ammonia monooxygenase and hydroxylamine oxidoreductase in the ammonia-oxidizing bacterium Nitrosomonas sp strain ENI-11

被引：13

作者：

Hirota, R ^{[1
]}

Yamagata, A ^{[1
]}

Kato, J ^{[1
]}

Kuroda, A ^{[1
]}

Ikeda, T ^{[1
]}

Takiguchi, N ^{[1
]}

Ohtake, H ^{[1
]}

机构：

[1] Hiroshima Univ, Dept Fermentat Technol, Higashihiroshima 7398527, Japan

来源：

JOURNAL OF BACTERIOLOGY | 2000年 / 182卷 / 03期

关键词：

D O I：

10.1128/JB.182.3.825-828.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Pulsed-field gel electrophoresis of PmeI digests of the Nitrosomonas sp. strain ENI-11 chromosome produced four bands ranging from 1,200 to 480 kb in size. Southern hybridizations suggested that a 487-kb PmeI fragment contained two copies of the amoCAB genes, coding for ammonia monooxygenase (designated amoCAB(1) and amoCAB(2)), and three copies of the hao gene, coding for hydroxylamine oxidoreductase (hao(1), hao(2), and hao(3)). In this DNA fragment, amoCAB(1) and amoCAB(2) were about 390 kb apart, while hao(1), hao(2), and hao(3) were separated by at least about 100 kb from each other. Interestingly, hao, and hao, were located relatively close to amoCAB(1) and amoCAB(2), respectively. DNA sequence analysis revealed that hao(1) and hao(2) shared 160 identical nucleotides immediately upstream of each translation initiation codon. However, hao(3) showed only 30% nucleotide identity in the 160-bp corresponding region.

引用

页码：825 / 828

页数：4

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