Vibrational echo experiments on red blood cells: Comparison of the dynamics of cytoplasmic and aqueous hemoglobin

被引：19

作者：

McClain, BL ^{[1
]}

Finkelstein, IJ ^{[1
]}

Fayer, MD ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

来源：

CHEMICAL PHYSICS LETTERS | 2004年 / 392卷 / 4-6期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/j.cplett.2004.05.080

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Ultrafast spectrally resolved stimulated vibrational echo experiments measure the dephasing of the CO stretching mode of hemoglobin-CO (Hb-CO) inside living human erythrocytes (red blood cells). A method is presented to overcome the adverse impact on the vibrational echo signal from the strong light scattering caused by the cells. The results are compared to experiments on Hb-CO aqueous solutions. It is demonstrated that the dynamics of the protein as sensed by the CO ligand are the same inside the erythrocytes and in aqueous solution, but differences in the absorption spectra show that the cell affects the protein's potential energy surface. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：324 / 329

页数：6

共 35 条

[1] Characterizing global substates of myoglobin [J].