Efficiency of a myogenic DNA vaccine is strictly dependent upon cellular localization of HIV-1Pr55gag

被引：7

作者：

Bojak, A ^{[1
]}

Wild, J ^{[1
]}

Wolf, H ^{[1
]}

Wagner, R ^{[1
]}

机构：

[1] Univ Regensburg, Inst Med Microbiol, D-93053 Regensburg, Germany

来源：

VACCINE | 2002年 / 20卷 / 15期

关键词：

DNA vaccine; tissue-specific; HIV-1;

D O I：

10.1016/S0264-410X(02)00082-8

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Most studies on DNA-based immunization have used viral promoters to drive antigen expression. Recently, the use of tissue-specific DNA vaccines has been favored regarding safety issues. In this study, we determined the impact of antigen localization and tissue-specific expression on the induction of humoral as well as cellular immune responses in a BALB/c mouse model, Thereby, we show that using the muscle-specific muscle creatine kinase (MCK) promoter/enhancer the efficiency of immune stimulation is strictly dependent on the ability of HIV-1 Pr55(gag) to be released from cells. By contrast, localization of Pr55(gag) and derivatives thereof plays only a minor role when antigen is constitutively expressed using the ubiquitous viral CMV promoter. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

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页数：5

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