The γ subunit in chloroplast F1-ATPase can rotate in a unidirectional and counter-clockwise manner

被引：57

作者：

Hisabori, T

Kondoh, A

Yoshida, M

机构：

[1] Tokyo Inst Technol, Resources Utilizat Res Lab, Midori Ku, Yokohama, Kanagawa 2268503, Japan

[2] Teikyo Univ, Biotechnol Res Ctr, CREST, Genet Programming Team 13,Miyamae Ku, Kawasaki, Kanagawa 2160001, Japan

来源：

FEBS LETTERS | 1999年 / 463卷 / 1-2期

关键词：

F-1-ATPase; rotation; gamma subunit; chloroplast; regulation;

D O I：

10.1016/S0014-5793(99)01602-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rotation of the gamma subunit in chloroplast F-1-ATPase (CF1) was investigated by using a single molecule observation technique, which is developed by Noji et al, to observe the rotation of a central gamma subunit portion in the alpha(3)beta(3)gamma sub-complex of F-1-ATPase from thermophilic Bacillus PS3 (TF1) during ATP hydrolysis [Noji, H. et al. (1997) Nature 386, 299-302], We used two cysteines of the gamma subunit (Cys-199 and Cys-205) of CF1-ATPase, which are involved in the regulation of this enzyme, to fis the fluorochrome-labeled actin filament, Then we successfully observed a unidirectional, counter-clockwise rotation of the actin filament with the fluorescent microscope indicating the rotation of the gamma subunit in CF1-ATPase, We conclude that the rotation of the gamma subunit in the F-1-motor is a ubiquitous phenomenon in all F-1-ATPases in prokaryotes as well as in eukaryotes. (C) 1999 Federation of European Biochemical Societies.

引用

页码：35 / 38

页数：4

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