Investigating intraspecific variation of Acidovorax avenae subsp citrulli using DNA fingerprinting and whole cell fatty acid analysis

被引：73

作者：

Walcott, RR ^{[1
]}

Langston, DB ^{[1
]}

Sanders, FH ^{[1
]}

Gitaitis, RD ^{[1
]}

机构：

[1] Univ Georgia, Coastal Plain Expt Stn, Tifton, GA 31793 USA

来源：

PHYTOPATHOLOGY | 2000年 / 90卷 / 02期

关键词：

Citrullus lanatus;

D O I：

10.1094/PHYTO.2000.90.2.191

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

To assess the diversity of Acidovorax avenae subsp, citrulli, 121 strains from watermelon, cantaloupe, and pumpkin were compared using pulse field gel electrophoresis of SpeI-digested DNA and gas chromatographic analysis of fatty acid methyl esters. Twenty-nine unique DNA fragments resulted from DNA digestion, and 14 distinct haplotypes were observed. Based on cluster analysis, two subgroups, I and II, were recognized, which accounted for 84.8% (eight haplotypes) and 15.2% (six haplotypes) of the strains, respectively. Results of cellular fatty acid analysis varied quantitatively and qualitatively for the A. avenae subsp. citrulli strains and supported the existence of the two subgroups. Group I includes strains from cantaloupe and pumpkin as well as the ATCC type strain, which was first described in the United States in 1978, whereas group II represents the typical watermelon fruit blotch-causing strains that appeared in the mainland United States in 1989. Knowledge of the two A. avenae subsp. citrulli groups may be useful in screening for watermelon fruit blotch resistance.

引用

页码：191 / 196

页数：6

共 30 条

[1] CHARACTERIZATION OF ERWINIA-CHRYSANTHEMI BIOVARS IN ALPINE WATER SOURCES BY BIOCHEMICAL-PROPERTIES, GLC FATTY-ACID ANALYSIS AND GENOMIC DNA FINGERPRINTING [J].

COTHER, EJ ;

BRADLEY, JK ;

GILLINGS, MR ;

FAHY, PC .

JOURNAL OF APPLIED BACTERIOLOGY, 1992, 73 (02) :99-107

[2] Intraspecific genomic variation within Xanthomonas albilineans, the sugarcane leaf scald pathogen [J].

Davis, MJ ;

Rott, P ;

Warmuth, CJ ;

Chatenet, M ;

Baudin, P .

PHYTOPATHOLOGY, 1997, 87 (03) :316-324

[3] MEASURES OF THE AMOUNT OF ECOLOGIC ASSOCIATION BETWEEN SPECIES [J].

DICE, LR .

ECOLOGY, 1945, 26 (03) :297-302

[4] GENOMIC RELATEDNESS OF XANTHOMONAS-CAMPESTRIS STRAINS CAUSING DISEASES OF CITRUS [J].

EGEL, DS ;

GRAHAM, JH ;

STALL, RE .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (09) :2724-2730

[5] INGRESS OF THE WATERMELON FRUIT BLOTCH BACTERIUM INTO FRUIT [J].

FRANKLE, WG ;

HOPKINS, DL ;

STALL, RE .

PLANT DISEASE, 1993, 77 (11) :1090-1092

[6] CHARACTERIZATION OF FATTY-ACID METHYL-ESTER CONTENT OF CLAVIBACTER-MICHIGANENSIS SUBSP MICHIGANENSIS [J].

GITAITIS, RD ;

BEAVER, RW .

PHYTOPATHOLOGY, 1990, 80 (04) :318-321

[7] RESISTANCE OF COMMERCIAL WATERMELON (CITRULLUS-LANATUS) TO PSEUDOMONAS-PSEUDOALCALIGENES SUBSP CITRULLI [J].

GOTH, RW ;

WEBB, RE .

PLANT DISEASE, 1981, 65 (08) :671-672

[8]

Hodge N. C., 1995, Phytopathology, V85, P1187

[9]

HOPKINS D, 1995, BACTERIAL FRUIT BLOT

[10]

HOPKINS D L, 1991, Phytopathology, V81, P1228

← 1 2 3 →