Lipofuscin- and melanin-related fundus autofluorescence visualize different retinal pigment epithelial alterations in patients with retinitis pigmentosa

被引:74
作者
Kellner, U. [1 ,4 ]
Kellner, S. [1 ,4 ]
Weber, B. H. F. [2 ]
Fiebig, B. [2 ]
Weinitz, S. [1 ]
Ruether, K. [3 ]
机构
[1] AugenZentrum Siegburg, D-53721 Siegburg, Germany
[2] Univ Regensburg, Inst Humangenet, Regensburg, Germany
[3] Charite Campus Rudolf Virchow, Augenklin, Berlin, Germany
[4] RetinaScience, D-301212 Bonn, Germany
关键词
fundus autofluorescence; near-infrared autofluorescence; retinal pigment epithelium; retinitis pigmentosa; VITELLIFORM MACULAR DYSTROPHY; IN-VIVO; MULTIFOCAL ELECTRORETINOGRAPHY; CONGENITAL AMAUROSIS; GEOGRAPHIC ATROPHY; JUNCTIONAL ZONE; GENE-MUTATIONS; USHER-SYNDROME; HUMAN RPE; FLUORESCENCE;
D O I
10.1038/eye.2008.280
中图分类号
R77 [眼科学];
学科分类号
100212 [眼科学];
摘要
Aims To compare melanin-related near-infrared fundus autofluorescence (FAF; NIA, excitation 787nm, emission >800nm) with lipofuscin-related FAF (excitation 488nm, emission >500nm) in retinitis pigmentosa (RP). Methods Thirty-three consecutive RP patients with different modes of inheritance were diagnosed clinically, with full-field ERG, and if possible with molecular genetic methods. FAF and NIA imaging were performed with a confocal scanning laser ophthalmoscope (Heidelberg Retina Angiograph 2). Results Rings of increased FAF were present within an area of preserved retinal pigment epithelium (RPE) at the posterior pole (31/33). Rings of increased NIA were located in the same region as rings of increased FAF. In contrast to FAF, NIA showed a precipitous decline of NIA peripheral to the ring. In larger areas of preserved NIA (11/31), pericentral and foveal NIA were of similar intensity with an area of lower NIA in between. In smaller areas of preserved NIA (20/31), NIA was homogeneous from the perifovea to the fovea. In one patient without a ring of increased FAF, NIA distribution was normal. In the remaining patient with severely advanced RP, no residual RPE as well as no FAF and NIA were detectable. Conclusion Characteristic features for FAF and NIA alterations in a heterogeneous group of RP patients indicate a common pathway of RPE degeneration. Patterns of NIA and FAF indicate different pathophysiologic processes involving melanin and lipofuscin. Combined NIA and FAF imaging will provide further insight into the pathogenesis of RP and non-invasive monitoring of future therapeutic interventions. Eye (2009) 23, 1349-1359; doi:10.1038/eye.2008.280; published online 12 September 2008
引用
收藏
页码:1349 / 1359
页数:11
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