Topical delivery of imiquimod to a mouse model as a novel adjuvant for human immunodeficiency virus (HIV) DNA

被引：46

作者：

Zuber, AK

Bråve, A

Engström, G

Zuber, B

Ljungberg, K

Fredriksson, M

Benthin, R

Isaguliants, MG

Sandström, E

Hinkula, J

Wahren, B ^{[1
]}

机构：

[1] Karolinska Inst, Microbiol & Tumorbiol Ctr, SE-17182 Stockholm, Sweden

[2] Swedish Inst Infect Dis Control, SE-17182 Stockholm, Sweden

[3] DI Ivanovskii Inst Virol, Moscow 1230987, Russia

[4] Soder Sjukhuset, Venhalsan, Stockholm, Sweden

来源：

VACCINE | 2004年 / 22卷 / 13-14期

关键词：

imiquimod; DNA vaccination; HIV-1; GM-CSF;

D O I：

10.1016/j.vaccine.2003.10.051

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We evaluated the compound imiquimod as a possible adjuvant for DNA immunization against human immunodeficiency virus (HIV). We found that gene-gun epidermal delivery of the DNA in combination with imiquimod resulted in the strongest HIV specific immune responses. The effect of imiquimod was further compared to that of recombinant granulocyte macrophage-colony stimulating factor (GM-CSF), a known DNA vaccine adjuvant. Both adjuvants were able to enhance the immune responses induced by the HIV-1 genes alone. The delivery of an adjuvant as a topical cream rather than through injections has a clear clinical benefit. We show for the first time that imiquimod can act as an adjuvant for DNA vaccination. (C) 2004 Elsevier Ltd. All rights reserved.

引用

收藏

页码：1791 / 1798

页数：8

相关论文

共 24 条

[1] Control of a mucosal challenge and prevention of AIDS by a multiprotein DNA/MVA vaccine [J].

Amara, RR ;

Villinger, F ;

Altman, JD ;

Lydy, SL ;

O'Neil, SP ;

Staprans, SI ;

Montefiori, DC ;

Xu, Y ;

Herndon, JG ;

Wyatt, LS ;

Candido, MA ;

Kozyr, NL ;

Earl, PL ;

Smith, JM ;

Ma, HL ;

Grimm, BD ;

Hulsey, ML ;

Miller, J ;

McClure, HM ;

McNicholl, JM ;

Moss, B ;

Robinson, HL .

SCIENCE, 2001, 292 (5514) :69-74

[2] Dose-response resistance to HIV-1/MuLV pseudotype virus ex vivo in a hairpin ribozyme transgenic mouse model [J].

Andäng, M ;

Hinkula, J ;

Hotchkiss, G ;

Larsson, S ;

Britton, S ;

Wong-Staal, F ;

Wahren, B ;

Ährlund-Richter, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (22) :12749-12753

[3] Immunogenicity of a novel DNA vaccine cassette expressing multiple human immunodeficiency virus (HIV-1) accessory genes [J].

Ayyavoo, V ;

Kudchodkar, S ;

Ramanathan, MP ;

Le, P ;

Muthumani, K ;

Megalai, NM ;

Dentchev, T ;

Santiago-Barrios, L ;

Mrinalini, C ;

Weiner, DB .

AIDS, 2000, 14 (01) :1-9

[4] Augmentation of immune responses to HIV-1 and simian immunodeficiency virus DNA vaccines by IL-2/Ig plasmid administration in rhesus monkeys [J].

Barouch, DH ;

Craiu, A ;

Kuroda, MJ ;

Schmitz, JE ;

Zheng, XX ;

Santra, S ;

Frost, JD ;

Krivulka, GR ;

Lifton, MA ;

Crabbs, CL ;

Heidecker, G ;

Perry, HC ;

Davies, ME ;

Xie, H ;

Nickerson, CE ;

Steenbeke, TD ;

Lord, CI ;

Montefiori, DC ;

Strom, TB ;

Shiver, JW ;

Lewis, MG ;

Letvin, NL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (08) :4192-4197

[5] Potent CD4+ T cell responses elicited by a bicistronic HIV-1 DNA vaccine expressing gp120 and GM-CSF [J].

Barouch, DH ;

Santra, S ;

Tenner-Racz, K ;

Racz, P ;

Kuroda, MJ ;

Schmitz, JE ;

Jackson, SS ;

Lifton, MA ;

Freed, DC ;

Perry, HC ;

Davies, ME ;

Shiver, JW ;

Letvin, NL .

JOURNAL OF IMMUNOLOGY, 2002, 168 (02) :562-568

[6] Therapeutic immunization of HIV-infected chimpanzees using HIV-1 plasmid antigens and interleukin-12 expressing plasmids [J].

Boyer, JD ;

Cohen, AD ;

Ugen, KE ;

Edgeworth, RL ;

Bennett, M ;

Shah, A ;

Schumann, K ;

Nath, B ;

Javadian, A ;

Bagarazzi, ML ;

Kim, J ;

Weiner, DB .

AIDS, 2000, 14 (11) :1515-1522

[7] Gene combination raises broad human immunodeficiency virus-specific cytotoxicity [J].

Calarota, SA ;

Kjerrström, A ;

Islam, KB ;

Wahren, B .

HUMAN GENE THERAPY, 2001, 12 (13) :1623-1637

[8] Early commitment of adoptively transferred CD4+ T cells following particle-mediated DNA vaccination:: implications for the study of immunomodulation [J].

Creusot, RJ ;

Thomsen, LL ;

van Wely, CA ;

Topley, P ;

Tite, JP ;

Chain, BM .

VACCINE, 2001, 19 (13-14) :1678-1687

[9] Mapping of B-cell epitopes in rabbits immunised with various gag antigens for the production of HIV-1 gag capture ELISA reagents [J].

Devito, C ;

Levi, M ;

Broliden, K ;

Hinkula, J .

JOURNAL OF IMMUNOLOGICAL METHODS, 2000, 238 (1-2) :69-80

[10] Small anti-viral compounds activate immune cells via the TLR7 MyD88-dependent signaling pathway [J].

Hemmi, H ;

Kaisho, T ;

Takeuchi, O ;

Sato, S ;

Sanjo, H ;

Hoshino, K ;

Horiuchi, T ;

Tomizawa, H ;

Takeda, K ;

Akira, S .

NATURE IMMUNOLOGY, 2002, 3 (02) :196-200