Human cytomegalovirus latent gene expression in granulocyte-macrophage progenitors in culture and in seropositive individuals

被引:221
作者
Kondo, K [1 ]
Xu, JK [1 ]
Mocarski, ES [1 ]
机构
[1] STANFORD UNIV, SCH MED, DEPT MICROBIOL & IMMUNOL, STANFORD, CA 94302 USA
关键词
herpesvirus; cDNA; leukocytes; fetal liver; peripheral blood;
D O I
10.1073/pnas.93.20.11137
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Following infection with cytomegalovirus, human granulocyte-macrophage progenitors carry the viral genome but fail to support productive replication. Viral transcripts arise from a region encompassing the major regulatory gene locus; however, their structure differs significantly from productive phase transcripts, One class, sense transcripts, is encoded in the same direction as productive phase transcripts but uses two novel start sites in the ie1/ie2 promoter/enhancer region, These transcripts have the potential to encode a novel 94 aa protein, The other class, antisense transcript, is unspliced and complimentary to ie1 exons 2-4, and has the potential to encode novel 154 and 152 aa proteins, Consistent with a role in latency, these transcripts are present in bone marrow aspirates from naturally infected, healthy seropositive donors but are not present in seronegative controls, Sense latent transcripts are present in a majority of seropositive individuals, Consistent with the expression of latent transcripts, antibody to the 94 aa and 152 aa proteins is detectable in the serum of seropositive individuals, Thus, latent infection by cytomegalovirus is accompanied by the presence of latency-associated transcripts and expression of immunogenic proteins, Overall, these results suggest that bone marrow-derived myeloid progenitors are an important natural site of viral latency.
引用
收藏
页码:11137 / 11142
页数:6
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