Efficient delivery of siRNA for inhibition of gene expression in postnatal mice

被引：449

作者：

Lewis, DL

Hagstrom, JE

Loomis, AG

Wolff, JA

Herweijer, H

机构：

[1] Mirus Corp, Madison, WI 53719 USA

[2] Univ Wisconsin, Waisman Ctr, Dept Pediat & Med Genet, Madison, WI 53705 USA

来源：

NATURE GENETICS | 2002年 / 32卷 / 01期

关键词：

D O I：

10.1038/ng944

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

It has recently been shown that RNA interference can be induced in cultured mammalian cells by delivery of short interfering RNAs (siRNAs). Here we describe a method for efficient in vivo delivery of siRNAs to organs of postnatal mice and demonstrate effective and specific inhibition of transgene expression in a variety of organs.

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收藏

页码：107 / 108

页数：2

相关论文

共 16 条

[1] Specific interference with gene expression induced by long, double-stranded RNA in mouse embryonal teratocarcinoma cell lines [J].

Billy, E ;

Brondani, V ;

Zhang, HD ;

Müller, U ;

Filipowicz, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (25) :14428-14433

[2] dsRNA-mediated gene silencing in cultured Drosophila cells:: a tissue culture model for the analysis of RNA interference [J].

Caplen, NJ ;

Fleenor, J ;

Fire, A ;

Morgan, RA .

GENE, 2000, 252 (1-2) :95-105

[3] Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems [J].

Caplen, NJ ;

Parrish, S ;

Imani, F ;

Fire, A ;

Morgan, RA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (17) :9742-9747

[4] RNA interference is mediated by 21-and 22-nucleotide RNAs [J].

Elbashir, SM ;

Lendeckel, W ;

Tuschl, T .

GENES & DEVELOPMENT, 2001, 15 (02) :188-200

[5] Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells [J].

Elbashir, SM ;

Harborth, J ;

Lendeckel, W ;

Yalcin, A ;

Weber, K ;

Tuschl, T .

NATURE, 2001, 411 (6836) :494-498

[6] Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans [J].

Fire, A ;

Xu, SQ ;

Montgomery, MK ;

Kostas, SA ;

Driver, SE ;

Mello, CC .

NATURE, 1998, 391 (6669) :806-811

[7] An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells [J].

Hammond, SM ;

Bernstein, E ;

Beach, D ;

Hannon, GJ .

NATURE, 2000, 404 (6775) :293-296

[8] Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA [J].

Liu, F ;

Song, YK ;

Liu, D .

GENE THERAPY, 1999, 6 (07) :1258-1266

[9] 'Green mice' as a source of ubiquitous green cells [J].

Okabe, M ;

Ikawa, M ;

Kominami, K ;

Nakanishi, T ;

Nishimune, Y .

FEBS LETTERS, 1997, 407 (03) :313-319

[10] Functional anatomy of a dsRNA trigger: Differential requirement for the two trigger strands in RNA interference [J].

Parrish, S ;

Fleenor, J ;

Xu, SQ ;

Mello, C ;

Fire, A .

MOLECULAR CELL, 2000, 6 (05) :1077-1087