Characterization of spirochetal isolates from arthropods collected in South Moravia, Czech Republic, using fatty acid methyl esters analysis

被引：14

作者：

Cechová, L

Durnová, E

Sikutová, S

Halouzka, J

Nemec, M

机构：

[1] Masaryk Univ, Fac Sci, Dept Microbiol, Brno 60200, Czech Republic

[2] Reg Inst Publ Hlth Immunol & Parasitol, Ctr Microbiol, Ostrava 70200, Czech Republic

[3] Acad Sci Czech Republ, Inst Vertebrate Biol, Dept Med Zool, Valtice 69142, Czech Republic

[4] Masaryk Univ, Fac Sci, Dept Comparat Anim Physiol & Gen Zool, Brno 61137, Czech Republic

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2004年 / 808卷 / 02期

关键词：

spirochetes; arthropods; fatty acid methyl esters;

D O I：

10.1016/j.chrombe.2004.05.014

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Aim of this study was to evaluate cellular fatty acid analysis for characterization of spirochetes. Strains were isolated from arthropods collected in South Moravia, Czech Republic. Fatty acid methyl esters (FAME) profile was determined for five Borrelia burgdorferi sensu lato (s.l.) strains isolated from Ixodes ricinus ticks, one "Spironema culicis" strain recovered from mosquito Culex pipiens and seven spirochetal strains (not identified yet) isolated from mosquitoes and blackflies. Analysis was performed using a gas chromatography column in conjunction with Microbial Identification System Sherlock (MIDI Inc., Newark, DE, USA). Results obtained on the basis of cluster analysis of FAME profiles showed, that the B. burgdorferi sensu lato isolates could be well separated from other spirochetal isolates. We recommended method used in this study as a useful tool for preliminary identification of spirochetes isolated from ticks and dipterans. (C)2004 Elsevier B.V. All rights reserved.

引用

页码：249 / 254

页数：6

共 31 条

[1]

[Anonymous], 2001, 101 MIDI

[2]

[Anonymous], 1994, CHEM METHODS PROKARY

[3]

THE SPIROCHETES - A DIFFERENT WAY OF LIFE [J].