Development of an Antibody Hapten-Chip System for Detecting the Residues of Multiple Antibiotic Drugs

被引:12
作者
Chen, Ailiang [1 ,2 ,3 ]
Wang, Guoqing [3 ,4 ]
Cao, Qin
Wang, Yan [3 ,4 ]
Zhang, Zhaohui [6 ]
Sun, Yimin [1 ,2 ,3 ]
Wang, Hui [3 ,4 ]
Xu, Chaoyi [6 ]
Zhou, Qi [6 ]
Han, Pei [3 ,4 ]
Liu, Miao [3 ,4 ]
Yang, Yang [3 ,4 ]
Xing, Wanli [1 ,2 ,3 ,4 ,5 ]
Mitchelson, Keith R. [1 ,3 ,4 ]
Cheng, Jing [1 ,2 ,3 ,4 ,5 ]
机构
[1] Tsinghua Univ, Sch Med, Med Syst Biol Res Ctr, Beijing 100084, Peoples R China
[2] Tsinghua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China
[3] Natl Engn Res Ctr Beijing Biochip Technol, Beijing 102206, Peoples R China
[4] CapitalBio Corp, Beijing 102206, Peoples R China
[5] Tsinghua Univ, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China
[6] Beijing Entry Exit Inspect & Quarantine Bur, Beijing 100026, Peoples R China
关键词
forensic sciences; antibiotics; residue; chip; immunoassay; sample extraction; PROTEIN CHIP; MICROARRAYS; SERODIAGNOSIS; MILK;
D O I
10.1111/j.1556-4029.2009.01049.x
中图分类号
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号
0301 ; 10 ;
摘要
The abuse of antibiotic drugs during animal production remains a worldwide problem and the subsequent detection of the residues of various drugs present at low concentrations in complex biological matrices poses significant analytical challenges. The present study outlines a practical biochip assay system to identify antibiotic residues in different animal tissue extracts. The system uses a simple but efficient multiresidue sample extraction procedure to isolate the antibiotic residues which were then identified directly using high-affinity monoclonal antibodies presented in a competitive immunoassay with conjugated antibiotic hapten-chips. The hapten-chip can analyze six samples each for eight antibiotics on a single chip within 3 h. The analytical results with both artificial positive standard samples and the incurred samples show that the antibody hapten-chip system has a comparable accuracy and a similar sensitivity to a standard ultra performance liquid chromatography-mass spectrometry (MS)/MS assay. In conclusion, an effective analytical screening system based on antibody hapten-chip was developed for detecting multiple antibiotic residues from multiple samples.
引用
收藏
页码:953 / 960
页数:8
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