Isolation and characterization of an extracellular polysaccharide from Pseudomonas caryophylli CFR 1705

被引：42

作者：

Sudhamani, SR

Tharanathan, RN ^{[1
]}

Prasad, MS

机构：

[1] Cent Food Technol Res Inst, Dept Biochem & Nutr, Mysore 570013, Karnataka, India

[2] Cent Food Technol Res Inst, Dept Food Microbiol, Mysore 570013, Karnataka, India

来源：

CARBOHYDRATE POLYMERS | 2004年 / 56卷 / 04期

关键词：

extracellular polysaccharide; Pseudomonas caryophylli; purification; characterization; structure;

D O I：

10.1016/j.carbpol.2004.03.008

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Extracellular polysaccharides were isolated from Pseudomonas caryophylli CFR 1705 grown on lactose containing medium. The major fraction (no.1) obtained on DEAE-cellulose chromatography was composed of rhamnose, mannose and glucose in the ratio 1:3.26:4.97, respectively, and having a molecular weight of 1.1x10(6) Da. Methylation followed by GC-MS analysis revealed it to be a highly branched 1,4-linked hexosan with mannose and glucose as the branch-off residues at positions C-2 and C-6 of the main chain. Rhamnose was essentially found as non-reducing terminal residue. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：423 / 427

页数：5

共 14 条

[1] Acetyl substitution of the O-specific polysaccharide caryophyllan from the phenol phase of Pseudomonas (Burkholderia) caryophylli [J].