An antibody specific for interleukin-6 reverses age-associated changes in spontaneous and induced cytokine production in mice

被引:17
作者
Gorczynski, RM
Cinader, B
Ramakrishna, V
Terzioglu, E
Waelli, T
Westphal, O
机构
[1] UNIV TORONTO,DEPT SURG,TORONTO,ON,CANADA
[2] UNIV TORONTO,DEPT IMMUNOL,TORONTO,ON,CANADA
[3] CLIN LA PRAIRIE,MONTREUX,SWITZERLAND
关键词
D O I
10.1046/j.1365-2567.1997.00327.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
A number of quantitative and qualitative changes in the pattern of cytokine production have been reported to accompany the process of ageing in laboratory animals and in human populations, including an increase in serum levels of interleukin-1 (IL-1) and IL-6, as well as increased concanavalin A (ConA)-stimulated production of IL-4, IL-10 and transforming growth factor-beta (TGF-beta), and decreased production of IL-2 from cultured spleen cells. Increased IL-1 and IL-6 production is a feature of splenic adherent cells and peritoneal exudate cells taken from aged mice and stimulated with lipopolysaccharide in vitro. We have asked whether the altered production of lymphocyte-derived cytokines (IL-4, IL-2, TGF-beta) is itself a function of a primary alteration in IL-1/IL-6 production (from macrophage/monocytes) by infusing monoclonal antibodies to these cytokines prior to harvesting cells from aged mice and stimulating the cells in vitro. Anti-IL-6, but not anti-IL-1, reversed the age-associated alteration in lymphocyte cytokine production. The general pattern of cytokine production in aged mice is of a type-2 cytokine type, and thus these data are consistent with the idea that increased production of IL-6 in aged animals is causally implicated in this age-associated polarization to type-2 cytokine production.
引用
收藏
页码:20 / 25
页数:6
相关论文
共 34 条
[1]  
Borghesi C, 1996, FUND CLIN IMMUNOL, V4, P55
[2]   Dysregulation between TH1 and TH2 T cell subpopulations in the elderly [J].
Cakman, I ;
Rohwer, J ;
Schutz, RM ;
Kirchner, H ;
Rink, L .
MECHANISMS OF AGEING AND DEVELOPMENT, 1996, 87 (03) :197-209
[3]   Cytokine production pathway in the elderly [J].
Caruso, C ;
Candore, G ;
Cigna, D ;
DiLorenzo, G ;
Sireci, G ;
Dieli, F ;
Salerno, A .
IMMUNOLOGIC RESEARCH, 1996, 15 (01) :84-90
[4]   CYTOKINES AND MACROPHAGES [J].
CAVAILLON, JM .
BIOMEDICINE & PHARMACOTHERAPY, 1994, 48 (10) :445-453
[5]   ANTI-CD3-INDUCED APOPTOSIS IN T-CELLS FROM YOUNG AND OLD MICE [J].
CHREST, FJ ;
BUCHHOLZ, MA ;
KIM, YH ;
KWON, TK ;
NORDIN, AA .
CYTOMETRY, 1995, 20 (01) :33-42
[6]   STEROIDS AS REGULATORS OF THE MAMMALIAN IMMUNE-RESPONSE [J].
DAYNES, RA ;
ARANEO, BA ;
HENNEBOLD, J ;
ENIOUTINA, E ;
MU, HH .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1995, 105 (01) :S14-S19
[7]  
DRIESSEN C, 1994, LYMPHOKINE CYTOK RES, V13, P15
[8]  
ENGWERDA CR, 1994, J IMMUNOL, V152, P3740
[9]  
ERNST DN, 1993, J IMMUNOL, V151, P575
[10]   INTERLEUKIN-12 [J].
GERMANN, T ;
RUDE, E .
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 1995, 108 (02) :103-112