Caveolin-1 is essential in the differentiation of human adipose-derived stem cells into hepatocyte-like cells via an MAPK pathway-dependent mechanism

被引：30

作者：

Guan, Yin ^{[1
,2
]}

Wang, Nan ^{[1
,2
,3
]}

Cui, Fenggong ^{[2
]}

Liu, Yang ^{[1
]}

Liu, Peng ^{[1
]}

Zhao, Jingyuan ^{[1
]}

Han, Chao ^{[1
]}

Li, Xiaoyan ^{[1
]}

Leng, Zhiqian ^{[1
]}

Li, Ying ^{[1
]}

Ji, Xiaofei ^{[1
]}

Zou, Wei ^{[2
]}

Liu, Jing ^{[1
]}

机构：

[1] Dalian Med Univ, Affiliated Hosp 1, Regenerat Med Ctr, 222 Zhongshan Rd, Dalian 116011, Liaoning, Peoples R China

[2] Liaoning Normal Univ, Coll Life Sci, Liaoning Key Labs Biotechnol & Mol Drug Res & Dev, 850 Huanghe Rd, Dalian 116029, Liaoning, Peoples R China

[3] 6 Hosp Dalian, Cent Lab, Dalian 116031, Liaoning, Peoples R China

来源：

MOLECULAR MEDICINE REPORTS | 2016年 / 13卷 / 02期

关键词：

human adipose-derived stem cells; Caveolin-1; hepatic differentiation; TISSUE IN-VITRO; LIVER-REGENERATION; THERAPEUTIC IMPLICATIONS; TRANSPLANTATION; VIVO; METABOLISM; FAILURE; BIOLOGY; INJURY;

D O I：

10.3892/mmr.2015.4743

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Human adipose-derived stem cells (hADSCs), widely present in the adult human body, are an emerging and attractive tool for the establishment of stem cell-based therapies for the treatment of liver disease. However, the mechanism underlying hADSCs hepatic differentiation remains to be elucidated. Caveolin-1 (Cav-1), a 21-24 kDa membrane structural protein, is important in liver regeneration and development. In the present study, fluorescence immunocytochemistry and western blotting were used to analyze the expression levels of Cav-1 and evaluate its effects on the hepatic differentiation of hADSCs. The results revealed that primary hADSCs preserved the ability to proliferate and differentiate into hepatocyte-like cells. As demonstrated by semiquantitative reverse transcription-polymerase chain reaction, hepatocyte-inducing factors significantly increased the expression of Cav-1 in a time-dependent manner, as indicated by increased expression levels of the albumin (ALB) and a-fetoprotein (AFP) markers. In addition the expression levels of ALB and HNF1A significantly decreased following small interfering RNA-mediated knockdown of Cav-1. The mitogen-activated protein kinase (MAPK) signaling pathway was activated during hepatic differentiation and inhibited following Cav-1 knockdown. These results suggested that Cav-1 may regulate the hepatocyte-like differentiation of hADSCs by modulating mitogen-activated protein kinase kinase/MAPK signaling. The results of the present study will provide experimental and theoretical basis for further clinical studies on stem cell transplantation in the treatment of liver disease.

引用

页码：1487 / 1494

页数：8

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