Antisense and chemical suppression of the nonmevalonate pathway affects ent-kaurene biosynthesis in Arabidopsis

被引:37
作者
Okada, K
Kawaide, H
Kuzuyama, T
Seto, H
Curtis, IS
Kamiya, Y
机构
[1] Tokyo Gakugei Univ, Dept Biol, Koganei, Tokyo 1848501, Japan
[2] RIKEN, Plant Sci Ctr, Wako, Saitama 3510198, Japan
[3] Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku, Tokyo 1130032, Japan
关键词
Arabidopsis; ent-kaurene; fosmidomycin; gibberellin; isoprenoids; nonmevalonate pathway;
D O I
10.1007/s00425-002-0762-0
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Transgenic plants of Arabidopsis thaliana (L.) Heynh. (ecotype Columbia) expressing the antisense AtMECT gene, encoding 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, were generated to elucidate the physiological role of the nonmevalonate pathway for production of ent-kaurene, the latter being the plastidic precursor of gibberellins. In transformed plants pigmentation and accumulation of ent-kaurene were reduced compared to wild-type plants. Fosmidomycin, an inhibitor of 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR), caused a similar depletion of these compounds in transgenic plants. These observations suggest that both AtMECT and DXR are important in the synthesis of isopentenyl diphosphate and dimethylallyl diphosphate and that ent-kaurene is mainly produced through the nonmevalonate pathway in the plastid.
引用
收藏
页码:339 / 344
页数:6
相关论文
共 34 条
[1]   Strong, constitutive expression of the Arabidopsis ACT2/ACT8 actin subclass in vegetative tissues [J].
An, YQ ;
McDowell, JM ;
Huang, SR ;
McKinney, EC ;
Chambliss, S ;
Meagher, RB .
PLANT JOURNAL, 1996, 10 (01) :107-121
[2]   Terpenoid biosynthesis from 1-deoxy-D-xylulose in higher plants by intramolecular skeletal rearrangement [J].
Arigoni, D ;
Sagner, S ;
Latzel, C ;
Eisenreich, W ;
Bacher, A ;
Zenk, MH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (20) :10600-10605
[3]  
BECHTOLD N, 1993, CR ACAD SCI III-VIE, V316, P1194
[4]   The Arabidopsis thaliana FPS1 gene generates a novel mRNA that encodes a mitochondrial farnesyl-diphosphate synthase isoform [J].
Cunillera, N ;
Boronat, A ;
Ferrer, A .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (24) :15381-15388
[5]   Analysis of the expression of CLA1, a gene that encodes the 1-deoxyxylulose 5-phosphate synthase of the 2-C-methyl-D-erythritol-4-phosphate pathway in Arabidopsis [J].
Estévez, JM ;
Cantero, A ;
Romero, C ;
Kawaide, H ;
Jiménez, LF ;
Kuzuyama, T ;
Seto, H ;
Kamiya, Y ;
León, P .
PLANT PHYSIOLOGY, 2000, 124 (01) :95-103
[6]  
GRAY JC, 1987, ADV BOT RES, V14, P25
[7]   ENT-KAURENE BIOSYNTHESIS IN GERMINATING BARLEY (HORDEUM-VULGARE L, CV HIMALAYA) CARYOPSES AND ITS RELATION TO ALPHA-AMYLASE PRODUCTION [J].
GROSSELINDEMANN, E ;
GRAEBE, JE ;
STOCKL, D ;
HEDDEN, P .
PLANT PHYSIOLOGY, 1991, 96 (04) :1099-1104
[8]   PLASTIDIC ISOPRENOID SYNTHESIS DURING CHLOROPLAST DEVELOPMENT - CHANGE FROM METABOLIC AUTONOMY TO A DIVISION-OF-LABOR STAGE [J].
HEINTZE, A ;
GORLACH, J ;
LEUSCHNER, C ;
HOPPE, P ;
HAGELSTEIN, P ;
SCHULZESIEBERT, D ;
SCHULTZ, G .
PLANT PHYSIOLOGY, 1990, 93 (03) :1121-1127
[9]   The CYP88A cytochrome P450, ent-kaurenoic acid oxidase, catalyzes three steps of the gibberellin biosynthesis pathway [J].
Helliwell, CA ;
Chandler, PM ;
Poole, A ;
Dennis, ES ;
Peacock, WJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (04) :2065-2070
[10]   Biosynthesis of terpenoids:: YgbB protein converts 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate to 2C-methyl-D-erythritol 2,4-cyclodiphosphate [J].
Herz, S ;
Wungsintaweekul, J ;
Schuhr, CA ;
Hecht, S ;
Lüttgen, H ;
Sagner, S ;
Fellermeier, M ;
Eisenreich, W ;
Zenk, MH ;
Bacher, A ;
Rohdich, F .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (06) :2486-2490