Association and insulin regulated translocation of hormone-sensitive lipase with PTRF

被引：46

作者：

Aboulaich, Nabila

Ortegren, Unn

Vener, Alexander V.

Stralfors, Peter ^{[1
]}

机构：

[1] Linkoping Univ, Dept Cell Biol, SE-58185 Linkoping, Sweden

[2] Linkoping Univ, Ctr Diabet Res, SE-58185 Linkoping, Sweden

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2006年 / 350卷 / 03期

关键词：

hormone-sensitive lipase; polymerase I and transcript release factor; adipocyte; human; insulin; translocation; protein complex; caveolae; lipid metabolism; transcriptional control;

D O I：

10.1016/j.bbrc.2006.09.094

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polymerase I and transcript release factor (PTRF) is in human adipocytes mainly localized at the plasma membrane. This localization was under control of insulin, which translocated PTRF to the cytosol and nucleus, indicating a novel role for PTRF in insulin transcriptional control. In the plasma membrane PTRF was specifically bound to a triacylglycerol-metabolizing subclass of caveolae containing hormone-sensitive lipase (HSL). In response to insulin PTRF was translocated to the cytosol in parallel with HSL. PTRF and HSL were quantitatively immunoprecipitated from the cytosol by antibodies against either PTRF or HSL. The findings indicate also a novel extra-nuclear function for PTRF in the control of lipolysis. (c) 2006 Elsevier Inc. All rights reserved.

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收藏

页码：657 / 661

页数：5

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