Non-polar distribution of green fluorescent protein on the surface of Autographa californica nucleopolyhedrovirus using a heterologous membrane anchor

被引：60

作者：

Chapple, SDJ ^{[1
]}

Jones, IM ^{[1
]}

机构：

[1] Univ Reading, Sch Anim & Microbial Sci, Reading RG6 6AH, Berks, England

来源：

JOURNAL OF BIOTECHNOLOGY | 2002年 / 95卷 / 03期

基金：

美国国家卫生研究院; 英国生物技术与生命科学研究理事会;

关键词：

baculovirus; VSV G protein; surface display; expression vector; membrane incorporation;

D O I：

10.1016/S0168-1656(02)00023-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Heterogeneous proteins can be displayed on the surface of the budded form of Autographa californica nucleopolyhedrovirus (AcMNPV) after fusion of the display protein to the AcMNPV major envelope glycoprotein, gp64. However, display is restricted to the poles of the virion and is relatively low level. To investigate the use of alternative membrane anchor sequences that would be compatible with virus surface display, we have constructed a display vector containing the gp64 signal peptide and a membrane anchor from the vesicular stomatitis virus (VSV) G glycoprotein. Introduction of a gene encoding green fluorescent protein (GFP) between these signals led to abundant display of GFP on the surface of insect cells and on recombinant budded virions. In addition, and in contrast to gp64 based fusion proteins, GFP was localized to the lateral virion surfaces. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：269 / 275

页数：7

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