Cocaine induces DNA damage in distinct brain areas of female rats under different hormonal conditions

被引：30

作者：

de Souza, Marilise F. ^{[1
]}

Goncales, Tierre A. ^{[1
]}

Steinmetz, Aline ^{[2
]}

Moura, Dinara J. ^{[2
]}

Saffi, Jenifer ^{[2
]}

Gomez, Rosane ^{[1
,3
]}

Barros, Helena M. T. ^{[1
]}

机构：

[1] Fed Univ Hlth Sci Porto Alegre UFCSPA, Lab Behav Neurosci, Porto Alegre, RS, Brazil

[2] Fed Univ Hlth Sci Porto Alegre UFCSPA, Lab Toxicol Genet, Porto Alegre, RS, Brazil

[3] Fed Univ Rio Grande Sul UFRGS, Inst Basic Hlth Sci, Dept Pharmacol, Porto Alegre, RS, Brazil

来源：

CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY | 2014年 / 41卷 / 04期

关键词：

cocaine; DNA damage; oestrogen; ovariectomy; progesterone; BEHAVIORAL SENSITIZATION; EXPRESSION;

D O I：

10.1111/1440-1681.12218

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

We evaluated levels of neuronal DNA damage after acute or repeated cocaine treatment in different brain areas of female rats after ovariectomy or sham surgery. Rats in the control and acute groups were given saline i.p., whereas in the repeated group were given 15mg/kg, i.p., cocaine for 8days. After a 10day washout period, the control group was given saline i.p., whereas rats in the acute and repeated groups were given a challenge dose of 15mg/kg, i.p., cocaine. After behavioural assessment, rats were killed and the cerebellum, hippocampus, hypothalamus, prefrontal cortex and striatum were dissected for the Comet assay. Acute cocaine exposure induced DNA damage in all brain areas. This effect persisted after repeated administration, except in the hypothalamus, where repeated treatment did not cause increased DNA damage. Sexual hormones exhibited a neuroprotective effect, decreasing cocaine-induced DNA damage in cycling rats in all brain areas.

引用

页码：265 / 269

页数：5

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