Nanoparticle-based optical detection of molecular interactions for DNA-chip technology

被引：8

作者：

Fritzsche, W ^{[1
]}

Csáki, A ^{[1
]}

Möller, R ^{[1
]}

机构：

[1] Inst Phys High Technol, Jena, Germany

来源：

BIOMEDICAL NANOTECHNOLOGY ARCHITECTURES AND APPLICATIONS | 2002年 / 4626卷

关键词：

DNA chip; nanoparticle; colloidal gold; optical detection; DNA;

D O I：

10.1117/12.472097

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We adapted the nanoparticle-labeling technique from microscopical applications for DNA-chip detection. Nanoparticles can be detected by simple optical means (e.g., reflection or transmission), and exhibit a high stability. So alternative optical readout devices. can be applied for the detection of specific DNA-binding on microstructured spots of complementary, surface-immobilized capture DNA. Several devices were tested, and the binding of gold-labeled DNA to complementary and non-complementary sequences was investigated using optical detection of the gold-labeled substrates before and after silver enhancement.

引用

收藏

页码：17 / 22

页数：6

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