Product quality of a recombinant fusion protein expressed in immobilised baby hamster kidney cells grown in protein-free medium

被引：4

作者：

Cruz, HJ

Dias, EM

Peixoto, CM

Moreira, JL

Carrondo, MJT ^{[1
]}

机构：

[1] IBET, ITQB, P-2781901 Oeiras, Portugal

[2] Univ Nova Lisboa, Fac Ciencias & Tecnol, P-2825 Monte De Caparica, Portugal

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 08期

关键词：

BHK cells; fusion protein; microcarriers; product quality; protein-free medium; tumour therapy;

D O I：

10.1023/A:1005631602517

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Baby hamster kidney cells producing a recombinant IgG-IL2 fusion protein were grown as spinner batch cultures in a protein-free medium, with cells immobilised in porous and non-porous supports at different support concentrations and agitation rates. Product quality, i.e., integrity, decreases up to 60% with the increase in agitation rate or support concentration and from porous to non-porous supports. LDH activity is a good indicator for the monitoring of product degradation, and thus product quality. Optimal conditions regarding titre of intact product were achieved at 60 rpm with 2 g porous support l(-1).

引用

页码：677 / 682

页数：6

共 15 条

[1] BIOLOGICAL RESPONSES OF HYBRIDOMA CELLS TO HYDRODYNAMIC SHEAR IN AN AGITATED BIOREACTOR [J].